FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281442

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281442
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences971835
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGCGCTCAGGCTGTTCATTTGCAGATACA41840.4305257579733185No Hit
GTGTATTACTGTGCGAGAGACTATTACGATGTTAGGACTGGTGTAACTCA36390.3744462794610196No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG24830.2554960461395196No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT23180.23851785539726394No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23110.23779756851728945No Hit
GAGTTACACCAGTCCTAACATCGTAATAGTCTCTCGCACAGTAATACACA22840.2350193191231022No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT20130.2071339270555187No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA20070.20651653830125485No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC18390.1892296531818673No Hit
GTATATGAACTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA17220.17719057247372239No Hit
GTGTAACTCAGTCCTACTACTTTGACTATTGGGGTCAGGGAACCCTGGTA16940.17430942495382445No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC16840.17328044369671808No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA16820.17307464744529677No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACGCTGCTGCCT16710.17194276806247974No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC16020.16484279738844557No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC15870.16329932550278597No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCAC15750.1620645479942583No Hit
GTTCATATACAGGCAGCAGCGTGGTTTTCGGCGGAGGGACCAAGCTGACC15460.15908050234864973No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC15180.15619935482875177No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG14420.14837909727474313No Hit
GTCCTACTACTTTGACTATTGGGGTCAGGGAACCCTGGTAACCGTCTCCT14280.1469385235147942No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGAAACCTATAACTATGTC14250.14662982913766226No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA14040.1444689684977388No Hit
CTCCTGTGCAGCCTCTGGATTCACCTTCAGCAATTATGCCATGCACTGGG13850.14251390410923664No Hit
GTATTACTGTGCGAGAGACTATTACGATGTTAGGACTGGTGTAACTCAGT13590.13983855284076No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT13540.1393240622122068No Hit
GGGCAGTGGTGGGTGCTTTATTTCCATGCTGGGTGCCTGGGAAGTATGTA13520.13911826596078553No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT13410.13798638657796847No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG13380.13767769220083656No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG13230.13613422031517697No Hit
GGTGTAACTCAGTCCTACTACTTTGACTATTGGGGTCAGGGAACCCTGGT12930.13304727654385776No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12870.13242988778959391No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT12780.13150380465819814No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT12650.13016612902395983No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12300.12656469462408743No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT11880.12224297334424053No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11810.12152268646426605No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11670.12008211270431708No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT11360.11689227080728724No Hit
GGGCTGGAGTGGGTGGCAGCTACATCATATGATGGAGGCAATAAATACTA11330.11658357643015534No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA11290.11617198392731276No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC11270.11596618767589148No Hit
GTCCTAACATCGTAATAGTCTCTCGCACAGTAATACACAGCCGTGTCCTC11200.115245900795917No Hit
GTATTTATTGCCTCCATCATATGATGTAGCTGCCACCCACTCCAGCCCCT10980.11298214203028292No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG10900.11215895702459779No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC10750.1106154851389382No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTG10640.10948360575612115No Hit
ATACACAGCCGTGTCCTCAGCGCTCAGGCTGTTCATTTGCAGATACAACG10530.1083517263733041No Hit
CTCCTGCACTGAAACCTATAACTATGTCTCCTGGTACCAACAACACCCAG10510.10814593012188282No Hit
TCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC10440.10742564324190834No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA10290.10588217135624875No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA10270.10567637510482747No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC10210.10505898635056364No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT10180.10475029197343169No Hit
CAGTAATACACAGCCGTGTCCTCAGCGCTCAGGCTGTTCATTTGCAGATA10120.10413290321916788No Hit
CAATAAATACTACGCAGACTCCGTGGAGGGCCGGTTCACCATCTCCAGAG10100.10392710696774658No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCACCTTCAGCAATTATGCC10090.10382420884203593No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10080.1037213107163253No Hit
GCTGTGTATTACTGTGCGAGAGACTATTACGATGTTAGGACTGGTGTAAC9830.10114885757355929No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGTTCATAT9810.10094306132213801No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC9760.10042857069358481No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC9750.10032567256787418No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)