FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281443

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281443
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722538
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGTTTTCAGGCTACTCAATTGCAAATATA32810.45409376392660317No Hit
GTGTATTACTGTGCGAGGAGAGGTGAGGGGGGGTCGGACTCCGAATACCT22960.31776875403092986No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG17620.24386260653418923No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC17620.24386260653418923No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15630.21632080250450497No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT15000.20760153791219282No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14850.2055255225330709No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14400.19929747639570514No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14320.19819026819350677No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC13910.19251582615724017No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13050.1806133379836078No Hit
GGTTATGATGTGCACTGGTACCAGCAACTTCCCGGAACAGCCCCCAAACT12500.17300128159349404No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGACCACTC12470.17258607851766966No Hit
GTACCTATGCTATCCACTGGGTCCGCCAGTCTCCAGGCAAGGGGCTGGAG12160.168295646734151No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11190.15487074728249586No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG11110.15376353908029752No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10560.14615148269018377No Hit
GTATTACTGTGCGAGGAGAGGTGAGGGGGGGTCGGACTCCGAATACCTCC10540.14587468063963419No Hit
CAGTAATACACAGCCGTGTCCTCAGTTTTCAGGCTACTCAATTGCAAATA10500.14532107653853502No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT10470.1449058734627106No Hit
ATACACAGCCGTGTCCTCAGTTTTCAGGCTACTCAATTGCAAATATAGCG10300.14255305603303908No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9850.1363250098956733No Hit
GTCATAGGACTGGCAGTAATAATCACCCTCATCCTCAGCCTGGAGCCCAG9800.13563300476929932No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC9680.13397219246600178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9580.13258818221325383No Hit
CTCCTGTGCAGCCTCTGATTTCACCTTCAGTACCTATGCTATCCACTGGG9550.13217297913742945No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT9440.1306505678594067No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9400.1300969637583075No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9180.12705214120226202No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9120.12622173505061327No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9080.1256681309495141No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8980.12428412069676613No Hit
CTCTTGCACTGGGAGCAGCTCCAACGGCGGGACAGGTTATGATGTGCACT8850.12248490736819378No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG8650.11971688686269787No Hit
TCCTATGACAGCAGGCTGAGTGGTCGTGTGGTATTCGGCGGAGGGACCAA8630.1194400848121483No Hit
ATGCTATCCACTGGGTCCGCCAGTCTCCAGGCAAGGGGCTGGAGTGGGTG8600.1190248817363239No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA8580.1187480796857743No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8570.11860967866049951No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT8330.11528805405390444No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG8310.11501125200335484No Hit
GGGCTGGAGTGGGTGGCAGTTATCTCTTATGATGGAAGTTATAAGTCCTA8180.11321203867478251No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8130.11252003354840852No Hit
GTCCTACGCAGACTCCGTTGAGGACCGATTCACCATCTCCAGAGACAATT8050.11141282534621016No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7780.10767599766379068No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7710.10670719048686712No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7690.10643038843631752No Hit
GCTGTGTATTACTGTGCGAGGAGAGGTGAGGGGGGGTCGGACTCCGAATA7560.10463117510774521No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7520.10407757100664601No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7450.10310876382972245No Hit
GAACACAGAGGACTCACCATGGACTTTGGGCTGAGCTGGGTTTTCCTCGT7380.10213995665279889No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7320.1013095505011501No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7230.10006394127367695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4200.095.510751
GTATCAA13800.081.495591
ATCAACG16300.069.353933
TCAACGC16650.067.858434
CAACGCA16950.066.6573945
TATCAAC17050.066.339952
AACGCAG17550.064.369596
CGCAGAG21300.053.3725938
GCAGAGT22850.049.7211469
TACATGG18550.035.118822
GTACATG18200.035.0293541
ACATGGG18600.035.0049973
CATGGGA8600.034.923024
TTAGCAC1501.2565542E-633.3893853
ATGGGAG6900.032.127255
ATAACGA900.003880434431.7817945
GTACACG8800.030.8789025
TACACGG8950.030.3571766
TATGTAC9750.028.6353472
GTATGTA10550.028.517431
Produced by FastQC (version 0.11.5)