FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences722538
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACACAGCCGTGTCCTCAGTTTTCAGGCTACTCAATTGCAAATATA29900.4138190655716378No Hit
GTGTATTACTGTGCGAGGAGAGGTGAGGGGGGGTCGGACTCCGAATACCT21790.3015758340737788No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC16570.22933049888033571No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15550.21521359430230658No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG15390.21299917789790987No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT13460.1862877800198744No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA13250.1833813584891037No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13100.18130534310998175No Hit
GGTTATGATGTGCACTGGTACCAGCAACTTCCCGGAACAGCCCCCAAACT12680.17549250004844036No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12550.173693286719868No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT12500.17300128159349404No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12370.1712020682649217No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC11090.1534867370297479No Hit
GTACCTATGCTATCCACTGGGTCCGCCAGTCTCCAGGCAAGGGGCTGGAG10650.14739709191765693No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG10490.1451826755132602No Hit
GTATTACTGTGCGAGGAGAGGTGAGGGGGGGTCGGACTCCGAATACCTCC9900.13701701502204727No Hit
ATACACAGCCGTGTCCTCAGTTTTCAGGCTACTCAATTGCAAATATAGCG9710.13438739554182616No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA9660.1336953904154522No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC9610.13300338528907824No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT9460.1309273699099563No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACGACCACTC9090.12580653197478886No Hit
CAGTAATACACAGCCGTGTCCTCAGTTTTCAGGCTACTCAATTGCAAATA9020.1248377247978653No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8840.12234650634291898No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8830.12220810531764419No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT8740.12096249609017103No Hit
CTCCTGTGCAGCCTCTGATTTCACCTTCAGTACCTATGCTATCCACTGGG8590.11888648071104911No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8560.1184712776352247No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8340.11542645507917923No Hit
GTCCTACGCAGACTCCGTTGAGGACCGATTCACCATCTCCAGAGACAATT8290.11473444995280524No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8230.11390404380115647No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7990.1105824191945614No Hit
TCCTATGACAGCAGGCTGAGTGGTCGTGTGGTATTCGGCGGAGGGACCAA7950.11002881509346221No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT7850.10864480484071425No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA7810.10809120073961508No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7510.10393916998137122No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7490.10366236793082165No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG7470.10338556588027205No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC7380.10213995665279889No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)