FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917764
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCAT33040.36000540443948553No Hit
TCCCTATGTATGGTACGGCAAACTACGCACAGAAATTCCAGGGCGGAGTC31610.3444240567291809No Hit
GTAGTACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG28210.30737749573964546No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC25090.2733818280080718No Hit
GGGTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT22960.2501732471528628No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGACCACTC21640.2357904646510432No Hit
CTCCTGCACTGGGAGAAGCTCCAACATCGGGGCAGGGTATGATGTACACT20920.22794531055914158No Hit
GTGTACTACTGTGCGAGAGTGGACTACGGTACTAACGGTTACTTTGACTA20150.21955535409974675No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA18430.20081415265798178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17950.19558404993004738No Hit
GTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCG17120.1865403306296608No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT16900.18414320021269084No Hit
GGCTATTGTCATAGGACTGGCAGTAATAATGAGCCTCATCCTCAGCCTGG16710.1820729512162168No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC15440.16823497108189034No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC15080.16431239403593953No Hit
TCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCCT14930.16267798693346003No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTACTA14710.16028085651649007No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT14690.1600629355694928No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14170.15439699094756384No Hit
ACATAGGGATGATCCCTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGT14090.1535253071595748No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13830.1506923348486103No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG13420.14622495543516634No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13410.14611599496166772No Hit
ACAATAGCCTGAGTGGTCATGTGGTATTCGGCGGAGGGACCAAGGTGACC13270.14459054833268684No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA13190.14371886454469776No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12820.13968732702524833No Hit
ATGTATGGTACGGCAAACTACGCACAGAAATTCCAGGGCGGAGTCACGAT12780.1392514851312538No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12570.1369633151877825No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT12500.13620059187329203No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG12470.13587371045279614No Hit
GTCATAGGACTGGCAGTAATAATGAGCCTCATCCTCAGCCTGGAGCCCAG12440.13554682903230025No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA12400.13511098713830572No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12370.1347841057178098No Hit
GTAATAATGAGCCTCATCCTCAGCCTGGAGCCCAGTGATGGCCAGGGAGG12200.13293177766833303No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA12120.13206009388034395No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT12010.130861528671859No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11970.13042568677786448No Hit
ACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGACCACTCA11740.12791959588739588No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG11580.12617622831141775No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11550.12584934689092186No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG11450.12475974215593552No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT11380.12399701884144507No Hit
TCCTATGACAATAGCCTGAGTGGTCATGTGGTATTCGGCGGAGGGACCAA11360.12377909789444781No Hit
CATTATTACTGCCAGTCCTATGACAATAGCCTGAGTGGTCATGTGGTATT11230.12236261173896558No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA11040.12029236274249153No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11030.1201834022689929No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC10770.11735042995802843No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT10610.11560706238205028No Hit
CCACATGACCACTCAGGCTATTGTCATAGGACTGGCAGTAATAATGAGCC10580.11528018096155439No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10530.11473537859406122No Hit
GTATGGTACGGCAAACTACGCACAGAAATTCCAGGGCGGAGTCACGATTA10400.11331889243857897No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10360.11288305054458445No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG10260.11179344580959812No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC10180.11092176202160903No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9960.10852463160463911No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG9950.10841567113114046No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC9910.10797982923714594No Hit
GTACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTG9850.10732606639615412No Hit
GTACTAACGGTTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTC9650.10514685692618146No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9330.10166012177422518No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG9310.10144220082722792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)