FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences917764
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCCTATGTATGGTACGGCAAACTACGCACAGAAATTCCAGGGCGGAGTC30020.3270993414428982No Hit
GTAGTACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGG29920.32600973670791183No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCGTCAT29190.3180556221425116No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC23740.2586721640857563No Hit
GTGTACTACTGTGCGAGAGTGGACTACGGTACTAACGGTTACTTTGACTA20990.228708033873632No Hit
GGGTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT20390.222170405463714No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC19380.211165397640352No Hit
CTCCTGCACTGGGAGAAGCTCCAACATCGGGGCAGGGTATGATGTACACT19280.21007579290536565No Hit
GTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCG16190.17640700659428785No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA15620.17019625960486576No Hit
GACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCACATGACCACTC15550.1694335362903753No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15100.1645303149829368No Hit
ACATAGGGATGATCCCTCCCATCCACTCAAGCCCTTGTCCAGGGGCCTGT14570.15875540988750922No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC14310.15592243757654473No Hit
GCCTACATGGAGCTGAGCAGCCTGAGATCTGAGGACACGGCCGTGTACTA14280.15559555615604884No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT13660.14884000679913353No Hit
TCCAGGAACAGCCCCCAAACTCGTCATCTATGGTAACAACAATCGGCCCT13350.1454622321206759No Hit
GCCTTGGGCTGACCTAGGACGGTCACCTTGGTCCCTCCGCCGAATACCAC12800.13946940607825103No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA12620.13750811755527564No Hit
ATGTATGGTACGGCAAACTACGCACAGAAATTCCAGGGCGGAGTCACGAT12570.1369633151877825No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12560.13685435471428384No Hit
ACAATAGCCTGAGTGGTCATGTGGTATTCGGCGGAGGGACCAAGGTGACC11920.1298808844103713No Hit
GTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCCCAGGGCAGAGGGTCAC11810.1286823192018863No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11470.12497766310293278No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG11380.12399701884144507No Hit
CATTATTACTGCCAGTCCTATGACAATAGCCTGAGTGGTCATGTGGTATT11350.12367013742094918No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11330.12345221647395192No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11050.12040132321599016No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG11010.11996548132199564No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG10990.11974756037499837No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT10840.11811315327251885No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10800.11767731137852433No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA10780.11745939043152705No Hit
GGCTATTGTCATAGGACTGGCAGTAATAATGAGCCTCATCCTCAGCCTGG10710.11669666711703662No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG10710.11669666711703662No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10620.11571602285554891No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA10580.11528018096155439No Hit
TCCTATGACAATAGCCTGAGTGGTCATGTGGTATTCGGCGGAGGGACCAA10310.11233824817709127No Hit
CTGCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGT10190.11103072249510768No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG10110.1101590387071186No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCA10020.1091783944456309No Hit
GTACACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCATGTAGGCTG9770.10645438260816506No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT9650.10514685692618146No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT9600.10460205455868829No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9530.10383933124419786No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG9380.10220492414171835No Hit
GTACTAACGGTTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTC9380.10220492414171835No Hit
GTATGGTACGGCAAACTACGCACAGAAATTCCAGGGCGGAGTCACGATTA9360.1019870031947211No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA9310.10144220082722792No Hit
GCACAGGGTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCT9310.10144220082722792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)