FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences706127
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTATTACTGTGCGAGAGTGGGGGGTTATTACCCCAGTTGGCACTACCT28990.4105493770950552No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGTTCATTTCCAGAAACA25260.35772601812421845No Hit
ATATTACGCAGACTCAGTGAAGGGCCGATTCACCATCTCCAGAGACAACG15330.21709975684260763No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT11850.16781683748107634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11850.16781683748107634No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC11470.16243536927493213No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11310.16016948792497665No Hit
ACCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCCTCC11130.15762037140627677No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT10940.15492963730320466No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10240.14501640639714952No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC9710.13751067442542206No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9260.13113788312867233No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT8740.12377376874131707No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG8710.12334891598820043No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG8330.11796744778205621No Hit
CTCCAGAGACAACGCCAAGAACTCACTGTTTCTGGAAATGAACAGCCTGA8200.11612641918521739No Hit
GATCAGCACTGAGCACCGAGGATTCACCATGGAACTGGGGCTCCGCTGGG7920.11216112682279533No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG7850.1111698037321898No Hit
TAGTAGTAGTGATTACATATATTACGCAGACTCAGTGAAGGGCCGATTCA7670.10862068721348991No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT7600.10762936412288442No Hit
GTATTACTGTCAGCAGTATGGTAGCTCACCTCAGACGTTCGGCCAAGGGA7590.10748774653851219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13550.074.0342251
GGTATCA6050.066.802031
CAACGCA15700.062.0262225
ATCAACG15700.062.0262223
TCAACGC15700.062.0262224
TATCAAC16150.061.1912422
AACGCAG17050.057.4484376
CGCAGAG19250.050.850478
GCAGAGT23250.042.721159
CAGCGCT4350.038.05629
TACACGG6950.036.2699476
GTACACG7000.035.0366135
CAGCCGT12150.034.3588459
ACACGGG7450.032.848047
TAACACG1153.2563318E-431.3626944
GTAATAG14350.031.1814841
AATAGAC13700.031.0650923
ACGGGGT8200.030.7214039
ATAGACA15050.030.6750074
ATACGGC950.00484474530.3722933
Produced by FastQC (version 0.11.5)