FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences717251
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTATTATACCAATCCCTACGCTATGGACGTCTGGGGCCAAGGGACCACGG16480.22976614880983087No Hit
ATAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTGTCAGAAATA13430.1872426807351959No Hit
TCCCTACGCTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCAT12650.17636782660463352No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT11540.16089207264960242No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA11450.1596372817883837No Hit
GTATAATAACGACTCCCAGAATCGTAATGAAGGGAGGCTCTCGCACAATA11440.15949786058158164No Hit
CCCTAAGACTCTCCTGTGCAGCCTCTGAATTCACCTTTAGTAATTATTGG11320.15782480609995664No Hit
GTATATTATTGTGCGAGAGCCTCCCTTCATTACGATTCTGGGAGTCGTTA10870.15155085179386296No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC10620.14806532162381092No Hit
GCCGTATATTATTGTGCGAGAGCCTCCCTTCATTACGATTCTGGGAGTCG10510.14653168834898803No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT10410.14513747628096718No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC10010.1395606280088839No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA9460.13189246163476942No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTTCATTGTCAGAAATAGTG9350.13035882835994653No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAATAGCAC8820.12296950439943619No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAGCCAGCAGTGATGTT8690.12115702871100913No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG8190.11418596837090501No Hit
CTCCAGAGACAACGCCAAGAACTCACTATTTCTGACAATGAACAGCCTGA8190.11418596837090501No Hit
CTCCTGCACTGGAGCCAGCAGTGATGTTGAGACTTATAACCTTGTCTCCT8110.11307059871648838No Hit
GCGTAGGGATTGGTATAATAACGACTCCCAGAATCGTAATGAAGGGAGGC8040.11209465026887379No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7870.1097244897532384No Hit
ATATTATTGTGCGAGAGCCTCCCTTCATTACGATTCTGGGAGTCGTTATT7700.10735432923760302No Hit
GTAATAATCAGCCTCGTCCTCAGTCTGGAGACCAGAGATTGTCAGGGAGG7680.10707548682399885No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7540.1051235899287697No Hit
GTATGTAGACGGGGTACGTGCCAAGCATCCTCGTGCGACCGCGAGAGCCC7410.10331111424034264No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10950.072.611671
TCAACGC11650.065.065354
CAACGCA11950.063.431915
GGTATCA4600.062.287331
ATCAACG12200.062.171113
AACGCAG13050.058.08116
TATCAAC13600.056.859152
CGCAGAG15950.047.5209058
ACGCAGA16750.045.2512477
GCAGAGT19450.039.3098039
CGAGTAG800.00217159235.7777143
GTACATG15000.029.1297071
TACATGG14500.028.6401672
CCTATAC2003.6476376E-728.622173
ACATGGG15050.027.576183
TAGACTC1659.2396396E-526.0038195
TCTAGAC1400.00107862825.5555113
CAGAGTA18050.024.9402059
TATACCA15700.024.1404885
TATTGAG6250.024.0275295
Produced by FastQC (version 0.11.5)