FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622993
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCATGTAGG21730.34880006677442604No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21200.3402927480726108No Hit
ATATATTACTGTGCGAGAGGGCCCCCGTATCAGTATGATAGTAATGGTTA20170.3237596570105924No Hit
GACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGGATGATCACTA18050.28973038220333136No Hit
GCCTTGGGCTGACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGG15280.24526760332780626No Hit
TTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCA14390.23098172852664475No Hit
GGTACCAACAGAGGCCAGGCCAGGCCCCTGTGTTGGTCATCTATTATGAT14190.22777141958256353No Hit
CCTATGTGGTGACTCAGTCACCCTCAGTGTCAGTGGCCCCGGGAAAGACG14120.22664781145213508No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA12830.20594131876281113No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA12790.20529925697399487No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC12300.1974340000609959No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT11810.18956874314799685No Hit
GTATGATAGTAATGGTTACCACAATTGGTTCGACCCCTGGGGCCAGGGAT11250.1805798781045694No Hit
CTACTACGATCCCACATCTGACAGAAATAGTCGGCCTCATCCCCGGCTTC10830.1738382293219988No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC10450.16773864232824445No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10410.1670965805394282No Hit
TCGTAGTAGTGATCATCCGGTGTTCGGCGGAGGGACCAAACTGACCGTCC10110.16228111712330637No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT9980.16019441630965356No Hit
GTGTTGGTCATCTATTATGATACCGACCGGCCCTCAGGGATCCCTGAGCG9970.16003390086244948No Hit
GCCATATATTACTGTGCGAGAGGGCCCCCGTATCAGTATGATAGTAATGG9860.1582682309432048No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9860.1582682309432048No Hit
ATATATGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCATGTAGGCTG9750.15650256102396012No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT9400.150884520371818No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG9370.1504029740302058No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG9350.15008194313579767No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT9300.14927936589977736No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA9240.148316273216553No Hit
GTATCATAATAGATGACCAACACAGGGGCCTGGCCTGGCCTCTGTTGGTA9170.14719266508612458No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA9070.14558751061408395No Hit
GTACATGGGAGTCTGGGCCGAAGGAAGCAGCACTGGTGGTGCCTCAGCCA9040.14510596427247177No Hit
GGCTCTGTGACCTCCTATGTGGTGACTCAGTCACCCTCAGTGTCAGTGGC8660.1390063772787174No Hit
ACCTAGGACGGTCAGTTTGGTCCCTCCGCCGAACACCGGATGATCACTAC8640.1386853463843093No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8560.1374012228066768No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGGTGACTCAGTCAC8500.13643813012345243No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA8480.1361170992290443No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT8420.13515400654581994No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA8400.1348329756514118No Hit
GCCTACATGGAGTTGAGCAGCCTGAGATCTGAGGACACGGCCATATATTA8350.1340303984153915No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT8090.1298569967880859No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8010.1285728732104534No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA7890.12664668784400468No Hit
ATATTACTGTGCGAGAGGGCCCCCGTATCAGTATGATAGTAATGGTTACC7860.1261651415023925No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA7850.12600462605518842No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7700.12359689434712749No Hit
TAGTAATGGTTACCACAATTGGTTCGACCCCTGGGGCCAGGGATCCCTGG7660.12295483255831126No Hit
GCACAGGCTCTGTGACCTCCTATGTGGTGACTCAGTCACCCTCAGTGTCA7660.12295483255831126No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA7640.12263380166390313No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC7610.12215225532229093No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT7410.1189419463782097No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT7320.11749730735337316No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC7290.11701576101176096No Hit
GGCCAGGCCCCTGTGTTGGTCATCTATTATGATACCGACCGGCCCTCAGG7100.1139659675148838No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC7060.11332390572606754No Hit
CTATCATACTGATACGGGGGCCCTCTCGCACAGTAATATATGGCCGTGTC7030.11284235938445536No Hit
TCTATTATGATACCGACCGGCCCTCAGGGATCCCTGAGCGATTCTCTGGC7000.11236081304284318No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG6860.11011359678198632No Hit
CCCCTGTGTTGGTCATCTATTATGATACCGACCGGCCCTCAGGGATCCCT6830.10963205044037412No Hit
CCCTCAGTGTCAGTGGCCCCGGGAAAGACGGCCAGGATTACCTGTGGGGG6760.1085084423099457No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA6730.10802689596833351No Hit
CCATTACTATCATACTGATACGGGGGCCCTCTCGCACAGTAATATATGGC6660.10690328783790508No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCAG6650.10674277239070103No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG6610.10610071060188477No Hit
CAGTAATATATGGCCGTGTCCTCAGATCTCAGGCTGCTCAACTCCATGTA6400.10272988621059947No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6340.10176679352737511No Hit
CCATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAAACGTGACTCGGCCC6250.10032215450253856No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6250.10032215450253856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG8850.056.9439133
GTATCAA8850.056.9439131
TATCAAC8750.056.7719232
TCAACGC8750.056.7719234
CAACGCA9950.048.4779555
CAGCGCT3650.047.3305139
AACGCAG10500.045.931266
GAATACG1101.05286745E-745.806619
GGCAGCG4150.041.628047
CGCAGAG12100.039.8577048
GCAGCGC4500.038.3903058
TACCGTC750.00153615738.39037
GTACATG16850.038.0261761
CCTATAC1001.4494116E-435.996693
TCTACAC6050.035.6991963
GCAGAGT13400.035.4537359
ACGCAGA13950.034.5719157
ACATGGG17150.034.0027053
CTAGACC1551.5462356E-632.5131383
CAGGGCA6050.032.1292764
Produced by FastQC (version 0.11.5)