FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681967
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13100.19209140618241058No Hit
CTCCTGCACTGGGGGCACCTCCAACATCGGGGCAGGTTATGATGTACACT11840.17361543887020925No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCGAACCACTC11620.1703894763236344No Hit
GTCTACATGGAGCTGAACAGCCTGAGATCTGACGACACGGCAGTCTATTT10870.1593918767330384No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT10590.15528610621921587No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10360.1519135090114331No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCT10060.1475144691751947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9830.14414187196741193No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9450.13856975484150993No Hit
CCCTCGAACCACTCAGGCTGCTGTCAGAGGACTGGCAGTAATAATCAGCC8940.13109138711990462No Hit
GGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACT8910.1306514831362808No Hit
GTCTGGGGCTGAGGTGAAGAAGCCGGGGTCCTCGGTGAAGGTCTCCTGCA8900.13050484847507282No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT8890.1303582138138649No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8820.12933177118540926No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA8810.12918513652420133No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT8620.12639907796125033No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC8480.12434619270433905No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT8480.12434619270433905No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8240.12082696083534833No Hit
GAAATAGACTGCCGTGTCGTCAGATCTCAGGCTGTTCAGCTCCATGTAGA7540.11056253455079205No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7500.10997599590596026No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC7280.10675003335938542No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG7240.10616349471455364No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT7080.10381734013522649No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)