FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281456

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281456
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences681967
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12850.1884255396522119No Hit
GTCTACATGGAGCTGAACAGCCTGAGATCTGACGACACGGCAGTCTATTT11270.16525726318135628No Hit
CTCCTGCACTGGGGGCACCTCCAACATCGGGGCAGGTTATGATGTACACT10840.15895197274941456No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT10430.15293995163988872No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC9950.1459014879019073No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA9850.14443514128982782No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCTCGAACCACTC9540.13988946679238146No Hit
GATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACTCCTCAT9430.13827648551909402No Hit
GTCTGGGGCTGAGGTGAAGAAGCCGGGGTCCTCGGTGAAGGTCTCCTGCA9140.13402408034406357No Hit
GGTTATGATGTACACTGGTACCAGCAACTTCCAGGAACAGCCCCCAAACT8730.12801205923453773No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC8430.12361301939829933No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCT8310.12185340346380397No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA8230.1206803261741404No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT8160.11965388354568476No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7640.1120288811628715No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC7540.11056253455079205No Hit
GAAATAGACTGCCGTGTCGTCAGATCTCAGGCTGTTCAGCTCCATGTAGA7310.10718993734300927No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7150.10484378276368211No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT7110.10425724411885033No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA7040.10323080149039471No Hit
CCCTCGAACCACTCAGGCTGCTGTCAGAGGACTGGCAGTAATAATCAGCC6910.10132455089469138No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8900.053.3986131
GGTATCA4850.050.4792941
TCAACGC9650.048.5022744
ATCAACG10000.046.80473
TATCAAC10500.044.5758972
CAACGCA10850.044.465295
AACGCAG11350.042.4940036
CAGCGCT3650.037.472229
TACACTA2705.456968E-1232.003215
TATTGAG7450.031.8958155
TCTACAC4050.028.44733
GTACATG18350.028.2535131
GCCTTAT2054.284011E-728.100381
GATTAGA1555.7593817E-527.8737641
CAGAGTA16700.027.587499
CGGGATA1050.00796753727.4313244
GTACACG5000.027.3627475
GCAGAGT17350.026.9688598
ACATGGG17700.026.8501513
AGGCCGT1907.557288E-626.5211966
Produced by FastQC (version 0.11.5)