FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1295754
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC36310.28022294355255706No Hit
GTAATACACAGCCGTGTCTTCGGGAGTCACAGAGTGCAGCTGCAGGGAGA36070.2783707401250546No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGATGATCACTA25010.19301503217431704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23080.1781202296114849No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG22970.17727130304054628No Hit
CCCATGGGGTGTCCTGTCACAGGTACAGCTGCAGCAGTCAGGTCCAGGAC20580.15882644390833445No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC20440.15774599190895802No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGG19960.15404158505395316No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT19590.15118610476988686No Hit
CCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGCCCCAGGACAGACG18850.14547514420175434No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA18830.14532079391612915No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT18210.14053593506174783No Hit
GGTATCAGCTGAAGCCAGGCCAGGCCCCTGTGGAGGTCATCTATTATGAT18120.13984135877643442No Hit
GTGTATTACTGTGCAAGGGGAGTCTATGATGGAACTACCTACCAGTTTGA17460.1347477993508027No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC16020.12363457878578805No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15470.11938994593109495No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG15160.11699751650390429No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA14660.11313875936327421No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14310.11043762936483313No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCGGATGATCACTAC14310.11043762936483313No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA14250.10997457850795753No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTGACTCAGCCAC14200.10958870279389453No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG14050.1084310756517055No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA13810.10657887222420305No Hit
TCTGGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG13790.10642452193857785No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA13710.10580712079607704No Hit
GTACATGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCA13700.10572994565326443No Hit
GGCTCTGTGACCTCCTATGTGCTGACTCAGCCACCCTCAGTGTCAGTGGC13630.10518971965357622No Hit
ATCATAGACTCCCCTTGCACAGTAATACACAGCCGTGTCTTCGGGAGTCA13420.10356904165451158No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT13310.10272011508357297No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)