FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences582722
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACA32390.5558396628237822No Hit
GTATATTACTGTGCCAACTTATTTACGGTGACTACGGCCTACTGGGGCCT25980.44583866749496326No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT20790.3567738990462004No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC17220.29550969415947914No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA16520.2834971049660044No Hit
ATATACGGCCGTGTCCTCGGCTCTCAGGCTGTCCATTTGCAGATACAACA15130.2596435349961045No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA15100.25912870974495555No Hit
GCCGTATATTACTGTGCCAACTTATTTACGGTGACTACGGCCTACTGGGG14940.25638297507216135No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14200.24368395221048803No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12990.22291933374748163No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC12960.22240450849633273No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG12790.21948716540648888No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT11310.1940891196831422No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT11040.18945569242280197No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10690.18344939782606456No Hit
GGGCTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT10430.17898757898277395No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA9880.16954911604504377No Hit
GTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCGGGGG9790.16800464029159703No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9400.1613119120266611No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9310.15976743627321432No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT9270.15908100260501576No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9140.15685009318337045No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT9070.15564883426402298No Hit
CTCCTGTGCAGCCTCTGGATTAACCTTTAGCAGCCAAGCCATGAGTTGGG8970.1539327500935266No Hit
GTACATGGGAGCTCTGAGAGAGGAGCCCAGCCCTGGGATTTTCAGGTGTT8780.15067219016958344No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG8660.14861288916498777No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG8520.1462103713262928No Hit
TTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGCT8490.1456955460751439No Hit
CTCTAGAGACAATTCCAAGAATATGTTGTATCTGCAAATGGACAGCCTGA8480.14552393765809427No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG8150.13986085989545616No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTAACCTTTAGCAGCCAAGCC8140.1396892514784065No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA8110.1391744262272576No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT8040.13797316730791012No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT8040.13797316730791012No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA7980.1369435168056123No Hit
CTATTAGTGGGAGTGGAGGTGAAGAATACTACGCGGACTCCGTGGAGGGC7850.13471260738396698No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC7830.13436939054986768No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC7510.12887792120427924No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7470.12819148753608067No Hit
TATCAGCACCATGGCCTGGACTCCTCTCTTTCTGTTCCTCCTCACTTGCT7450.12784827070198138No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT7440.12767666228493177No Hit
ATATTACTGTGCCAACTTATTTACGGTGACTACGGCCTACTGGGGCCTGG7420.12733344545083247No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC7330.12578896969738573No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAATACCGG7280.12493092761213752No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7260.12458771077803824No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG7000.12012589193474761No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG6970.11961106668359871No Hit
GTATTCTTCACCTCCACTCCCACTAATAGTTGAGACCCACTCCAGCCCCT6700.11497763942325842No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC6590.1130899468357124No Hit
GTAATAGTCAGCCTCATCCTCAGGCTGCGCATCCGAAAGGGTCAGGGCAG6560.11257512158456347No Hit
GGTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTT6550.11240351316751385No Hit
TCTCTAGAGACAATTCCAAGAATATGTTGTATCTGCAAATGGACAGCCTG6500.11154547108226565No Hit
CTCCACGGCGATGTCGCTGGGATAGAAGCCTTTGACCAGGCAGGTCAGGC6470.11103064583111671No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6440.11051582057996781No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6420.11017260374586854No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT6380.10948617007766996No Hit
TTTTAAAAGGTGTCCAGTGTGAGGTGCAGCTGTTGGAGTCTGGGGGAGGC6380.10948617007766996No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA6310.10828491115832249No Hit
GACTACGGCCTACTGGGGCCTGGGAACCCTGGTCACCGTCTCCTCAGCCT6160.1057107849025779No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA6120.10502435123437934No Hit
ATTAGTGGGAGTGGAGGTGAAGAATACTACGCGGACTCCGTGGAGGGCCG6070.10416630914913115No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6070.10416630914913115No Hit
GAAGAATACTACGCGGACTCCGTGGAGGGCCGGTTCACCATCTCTAGAGA6060.1039947007320815No Hit
CCTATGGTGCTGCTCGGCCGGTATTCGGCGGAGGGACCAAACTGACCGTC6030.10347987548093258No Hit
TCCTCAGGCTGCGCATCCGAAAGGGTCAGGGCAGCTTTGCCCCCAAGGAG5990.10279344181273403No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC5950.10210700814453548No Hit
CACTAATAGTTGAGACCCACTCCAGCCCCTTTCCTGGAGCCTGGCGGACC5910.10142057447633693No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC5880.100905749225188No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC5850.10039092397403908No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA5850.10039092397403908No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA5830.1000477071399398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8900.052.6128431
ATCAACG8950.051.4874733
TCAACGC9000.051.2014354
CAACGCA9100.050.6387835
TATCAAC9450.048.7632682
TACCCGC450.00990984647.9931076
AACGCAG9700.047.498336
GGCAGCG3800.047.3616187
CGCAGAG10000.046.073388
GCAGCGC4000.044.9935388
GCAGAGT11450.040.867499
GGTATCA3900.036.9431421
TTATTGA4200.036.001014
CAGCGCT5050.035.6384479
TATTGAG4350.034.7595985
TCTATAC1459.171508E-734.7595943
TATCACC900.003751493532.0008964
ATTGAGT3150.031.9954036
AATATAC17400.030.2077453
TATACGG16050.030.056925
Produced by FastQC (version 0.11.5)