FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873022
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCTCAGATCTCAGGCTGCTCAGCTCCAAGTAGG14220.1628824932246839No Hit
GTATATTACTGTGCGCGGAGATATTGTATTAGTACCAACTGCCCGGGCCA12020.13768267008162452No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT12010.13756812543097424No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA11170.12794637477635157No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10000.063.2929651
TCAACGC9550.062.4810184
ATCAACG9950.059.9692153
CAACGCA10050.059.3725055
AACGCAG10700.056.4117476
CGCAGAG13300.045.4699488
ACGCAGA13800.042.779097
GTACATG21000.030.8244971
TACATGG21300.030.3798832
TATCAAC20650.028.8988972
ACATGGG21450.028.4882013
ATACGAG1500.001569361823.9636045
GTGCTAC4303.6379788E-1223.4170591
TAGACTG4104.1836756E-1122.7946475
GTATATT14550.022.7388041
GCAGAGT26650.022.4221739
TATACGG10000.021.5672425
TATATTA16550.021.2873342
TTAGACC2053.8808663E-421.0412124
ATATACG11050.020.8190574
Produced by FastQC (version 0.11.5)