FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281471

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281471
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612795
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCAGGTCTCAGGCTGTTCATTTGCAGATACA42420.6922380241353144No Hit
GTCTATTACTGTGCGAGAGAGAGTTCTTGGGGGACGGTGGTGACTGCCAC37700.6152138969802299No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG18080.29504157181439145No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA17220.2810075147479989No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC14930.24363775814097702No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT14710.24004765051934168No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT14630.23874215683874705No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT14570.23776303657830106No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC14070.22960370107458447No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA13050.2129586566470027No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12970.21165316296640801No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACAGCACTCAGGCTG11460.18701196974518394No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG11330.18489054251421763No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG11130.181626808312731No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT10170.1659608841455952No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9750.15910704232247325No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT9420.1537218808900203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9240.15078452010868235No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGTAACTATGCTATGCACTGGA9210.15029495997845935No Hit
GCCTGAGACCTGACGACACGGCTGTCTATTACTGTGCGAGAGAGAGTTCT9110.14866309287771604No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC9110.14866309287771604No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA8890.14507298525608076No Hit
CTCCTGCACTGGGAGCAGCTCCAATCTCGGGGCAGGTTATGATGTACACT8860.14458342512585776No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT8740.1426251846049658No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACAGC8440.13772958330273583No Hit
GGGCTGGAGTGGGTGGCAGTTATGTCATATGATGGAAGTCAAAAATACTA8400.1370768364624385No Hit
ATGCTATGCACTGGATCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG8330.13593452949191817No Hit
GCTATGCACTGGATCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC8270.13495540923147217No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA8260.13479222252139786No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC7980.13022299463931658No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG7960.1298966212191679No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG7850.12810156740835027No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT7780.12695926043782996No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG7730.1261433268874583No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA7730.1261433268874583No Hit
GTACATGGGAGCTCTGGGAGACGAGCCCAGCACTGGAAGTCGCCGGTGTT7700.1256537667572353No Hit
CTATTACTGTGCGAGAGAGAGTTCTTGGGGGACGGTGGTGACTGCCACGT7670.1251642066270123No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC7630.12451145978671498No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT7460.12173728571545134No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC7260.1184735515139647No Hit
CCCTGAGACTCTCCTGTGCAGCCTCTGGATTCATCTTCAGTAACTATGCT7260.1184735515139647No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7190.11733124454344437No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT7170.11700487112329572No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT7160.11684168441322137No Hit
TCCTTTGACTATTGGGGCCGGGGAACCCTGGTCACCGTCTCCTCAGCCTC7140.11651531099307272No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC7060.11520981731247806No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGGCCCT7060.11520981731247806No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT6950.11341476350166042No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC6880.11227245653114011No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC6730.10982465588002513No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT6630.1081927887792818No Hit
GATCAGCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGG6590.10754004193898449No Hit
CAGTAATAGACAGCCGTGTCGTCAGGTCTCAGGCTGTTCATTTGCAGATA6500.10607136154831551No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA6430.1049290545777952No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT6430.1049290545777952No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG6300.10280762734682887No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA6240.10182850708638287No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC6230.10166532037630856No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC6210.1013389469561599No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT6180.1008493868259369No Hit
CTCCAGAGACAATTCCAGGAATACGCTGTATCTGCAAATGAACAGCCTGA6150.1003598266957139No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)