FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281472

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281472
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612795
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACAGCCGTGTCGTCAGGTCTCAGGCTGTTCATTTGCAGATACA40930.667923204334239No Hit
GTCTATTACTGTGCGAGAGAGAGTTCTTGGGGGACGGTGGTGACTGCCAC36370.5935100645403438No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCG16180.26403609690026847No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT15690.2560399481066262No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14730.24037402393949037No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT13360.21801744465930695No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT13030.212632283226854No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC12670.20675756166417805No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC12040.1964767989294952No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC11870.19370262485823153No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA11780.19223394446756256No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA10100.16481857717507487No Hit
GTCCTGGGCCCAGTCTGTGCTGACGCAGCCGCCCTCAGTGTCTGGGGCCC9610.15682242838143262No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG9360.15274276062957431No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG9340.15241638720942566No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT9280.15143726694897966No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG9070.14801034603741872No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT8700.14197243776466845No Hit
CTCCTGTGCAGCCTCTGGATTCATCTTCAGTAACTATGCTATGCACTGGA8540.13936145040347914No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT8370.1365872763322155No Hit
GACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACAGCACTCAGGCTG8230.13430266239117486No Hit
GATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACTCCTCAT8140.1328339820005059No Hit
CTCCTGCACTGGGAGCAGCTCCAATCTCGGGGCAGGTTATGATGTACACT7970.13005980792924224No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG7840.12793838069827593No Hit
ATGCTATGCACTGGATCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTG7710.12581695346730962No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC7710.12581695346730962No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT7620.12434827307664063No Hit
GCTATGCACTGGATCCGCCAGGCTCCAGGCAAGGGGCTGGAGTGGGTGGC7400.12075816545500534No Hit
GGTTATGATGTACACTGGTACCAGCAGCTTCCAGGAACAGCCCCCAAACT7380.12043179203485668No Hit
GCCTGAGACCTGACGACACGGCTGTCTATTACTGTGCGAGAGAGAGTTCT7350.11994223190463368No Hit
GGGCTGGAGTGGGTGGCAGTTATGTCATATGATGGAAGTCAAAAATACTA7330.11961585848448501No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG7030.11472025718225508No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA6990.11406751034195775No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA6880.11227245653114011No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT6840.11161970969084278No Hit
GTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGGCGTGGTCCAGCC6830.11145652298076844No Hit
GCCTTGGGCTGACCTAGGACGGTCAACTTGGTCCCTCCGCCGAACACAGC6640.10835597548935615No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC6630.1081927887792818No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC6570.10721366851883583No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC6510.10623454825838984No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG6430.1049290545777952No Hit
TCCAGGAACAGCCCCCAAACTCCTCATCTATGGTAACAACAATCGGCCCT6420.10476586786772085No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC6400.1044394944475722No Hit
GAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTCCTCGT6400.1044394944475722No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC6300.10280762734682887No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT6180.1008493868259369No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT6140.10019663998563957No Hit
CTATTACTGTGCGAGAGAGAGTTCTTGGGGGACGGTGGTGACTGCCACGT6130.10003345327556523No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)