FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences734286
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATACATAGCCGTGTCCTCAGGTCTCAGGCTGTCCACGTCCAGATACA14810.20169252852430797No Hit
ATGTATTACTGTGCGAGACCTCCCGGAAGCCTACGGCTATGGTTAATGGA13110.17854078656000522No Hit
GAGTTATAGTATCCCACTCACTTTCGGCGGGGGGACGAAGGTGGAGATCA11420.15552523131313956No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9290.1265174604990426No Hit
AATAAATACTACGCAGACTCCCTGAAGGGCCGATTCACCATCTCCAGAGA9140.12447465973748648No Hit
CTATAACTCTGTTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACT8960.12202329882361913No Hit
TTTAAGAGGTGTCCAGTGTCAGGTGCAGCTGGTGGAGTCTGGGGGAGACG8690.1183462574528181No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT7730.10527233257885893No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9150.054.3185731
TCAACGC9650.049.254764
ATCAACG9800.048.500863
CAACGCA10100.046.3472065
AACGCAG11000.042.5406656
TATCAAC11400.041.693722
CGCAGAG11350.041.2288368
GTACATG18000.036.0155751
TACATGG18050.033.1155852
GCAGAGT14550.032.1613279
ACATGGG19100.030.1639463
GGTATCA8950.028.9734251
TTTCGCG1000.006285708428.7967579
TAACCGT1000.006285708428.7967577
GTAATAC9950.027.5093861
GTACACG3607.2759576E-1226.005935
GTTATAG10300.025.8699783
TACACGG3659.094947E-1225.640956
TAGAGTG2551.0113399E-725.4175625
ATAGTAT11350.025.3715926
Produced by FastQC (version 0.11.5)