FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281478

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281478
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1345487
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT59820.4445973836982446No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC44370.3297690724622386No Hit
CTATGATAGTCTCCCTCGGACTTTCGGCGGAGGGACCAAGGTGGACATCA35560.2642909221716746No Hit
ATATAAATTGGTATCAGCAGAAACCAGGGAAAGCCCCTAAGCTCCTGATC34990.26005453787364724No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT33610.24979802852052824No Hit
GTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATTTCCAT30640.22772423665185915No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC29000.21553534147858733No Hit
ATCATAGTGTTGACAGTAGTATGTCGCAAAATCTTCAGGCAGCAGGCTGC28410.2111503121174712No Hit
GTAATAAACAGCCGTGTCCTCGGCTCGCAGGCTGTTCATTTGCAGATACA26260.19517096783543803No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT25830.19197509897903137No Hit
ATACTACTGTCAACACTATGATAGTCTCCCTCGGACTTTCGGCGGAGGGA25240.1875900696179153No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG25160.18699548936556057No Hit
CACTATGATAGTCTCCCTCGGACTTTCGGCGGAGGGACCAAGGTGGACAT24880.18491445848231902No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG24180.17971188127421522No Hit
ATGATAGTCTCCCTCGGACTTTCGGCGGAGGGACCAAGGTGGACATCAAA23910.17770517292251803No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG23430.17413769140838967No Hit
GTTTATTACTGTGTGAATTCTGGTGGGAGTTACTCTGAGTACTGGGGCCT22890.17012427470499528No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG22850.16982698457881792No Hit
CTATCATAGTGTTGACAGTAGTATGTCGCAAAATCTTCAGGCAGCAGGCT21310.15838131472098949No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA21270.15808402459481216No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT21200.15756376687400175No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG21130.15704350915319137No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19730.14663835473698372No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19560.1453748717007299No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC19500.14492893651146388No Hit
TCTAGATTCAGTGGAAGTGGAGCTGGAACAGATTTCACTTTCACCATCAG19480.1447802914483752No Hit
ACATACTACTGTCAACACTATGATAGTCTCCCTCGGACTTTCGGCGGAGG19160.1424019704389563No Hit
AGTCAGGACATTTCCATCTATATAAATTGGTATCAGCAGAAACCAGGGAA17490.12999010767105146No Hit
CTACTGTCAACACTATGATAGTCTCCCTCGGACTTTCGGCGGAGGGACCA17260.12828068944553161No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA17010.12642262615692312No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG16990.12627398109383442No Hit
GATCTACGATGCATCCAAGTTGGAAACAGGGGTCCCATCTAGATTCAGTG16800.12486185299449196No Hit
CATCTAGATTCAGTGGAAGTGGAGCTGGAACAGATTTCACTTTCACCATC16590.1233010798320608No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC16230.12062546869646455No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG15870.11794985756086829No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15850.11780121249777961No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGG15490.11512560136218335No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC15280.11356482819975221No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC15190.11289592541585314No Hit
ACTGTCAACACTATGATAGTCTCCCTCGGACTTTCGGCGGAGGGACCAAG14930.1109635395957003No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14780.10984870162253518No Hit
GTAGTATGTCGCAAAATCTTCAGGCAGCAGGCTGCTGATGGTGAAAGTGA14510.10784199327083799No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14200.10553799479296344No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA14020.1042001892251653No Hit
TCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGA13930.10353128644126626No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT13700.10182186821574642No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC13490.10026109505331528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19450.069.933711
TCAACGC20200.065.853144
CAACGCA20800.064.3040165
ATCAACG20700.063.9056053
AACGCAG22450.058.9504286
TATCAAC24600.053.4839672
CGCAGAG25400.051.8206528
ACGCAGA29000.045.635767
GCAGAGT30850.042.8990979
GAATACG3900.036.8850949
CATGGGA22200.036.276154
GTACATG39900.036.2549671
TACATGG38550.035.994872
ACATGGG39500.035.491883
CAGCGCT5800.033.482769
GGCAGCG6050.030.9103187
TACACGG9100.029.2446126
GCAGCGC6950.028.9773568
GTACACG9000.028.7639475
CAGCCGT14050.028.156069
Produced by FastQC (version 0.11.5)