FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281479

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281479
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106506
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATACGGCCGTGTCCGCGGCGGTCACAGATATCAGCCTCAGGGAGA52640.4757317176770844No Hit
GTATATTACTGTGCGAGAGCCCTTAAGAGGATAGCACCAGCTGGGAAAGG52170.4714841130549676No Hit
GTCTCTGGTGACTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG46420.41951873735885753No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA33820.3056467836595554No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33560.30329704493242693No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT32800.2964285778838976No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30430.27500980564045746No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC30070.2717563212490488No Hit
GCCGTATATTACTGTGCGAGAGCCCTTAAGAGGATAGCACCAGCTGGGAA29550.2670568437947919No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC28810.2603691258791186No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA28720.2595557547812664No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG25630.23163001375500902No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG24100.21780270509152233No Hit
CCTACAACCCGTCTCTCACGCCTCGACTCACCATATCAGTAGACACGTAC23710.21427809700082961No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTACTACTGGG21540.19466681608594982No Hit
ATATACGGCCGTGTCCGCGGCGGTCACAGATATCAGCCTCAGGGAGAATT20810.18806947273670455No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20280.1832796207160196No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20240.18291812245030756No Hit
CTTTTATAGTGGGAGCACCTCCTACAACCCGTCTCTCACGCCTCGACTCA20040.1811106311217472No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18930.17107905424823724No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC18300.16538545656327214No Hit
ATATTACTGTGCGAGAGCCCTTAAGAGGATAGCACCAGCTGGGAAAGGAG17890.1616800993397234No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT17830.16113785194115532No Hit
CCATATCAGTAGACACGTACAAGAACCAATTCTCCCTGAGGCTGATATCT17040.15399826119334192No Hit
TCTGGGGCCAGGGGGCCACGGTCACCGTCTCCTCAGCCTCCACCAAGGGC17010.1537271374940579No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT16920.1529137663962057No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA16630.1502929039697932No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG16440.14857578720766088No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC15920.14387630975340396No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15860.14333406235483584No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA15810.14288218952269577No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT15790.14270144038983973No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG15710.1419784438584156No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG15680.14170732015913154No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC15450.1396287051312871No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15410.13926720686557506No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT15260.13791158836915482No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC15210.1374597155370147No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC15030.1358329733413104No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT14900.13465810397774616No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14560.13158536871919355No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT14430.13041049935562934No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC13990.12643401843279659No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCT13820.12489765080352026No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13720.1239939051392401No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT13620.12309015947495991No Hit
AGTTACTACTGGGGCTGGATCCGACAGCCCCCAGGGAAGGGACTGGAGTG13510.12209603924425172No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13320.1203789224821194No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC13000.11748693635642282No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA12840.11604094329357455No Hit
GTCCCAACTACAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGG12710.11486607393001033No Hit
GGATTGGGAATATCTTTTATAGTGGGAGCACCTCCTACAACCCGTCTCTC12430.11233558607002583No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC12420.1122452115035978No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG12340.11152221497217367No Hit
TAATTATGTATCCTGGTACCAGCAGCTCCCAGGATCAGCCCCCAAACTCC12270.11088959300717754No Hit
CACCATATCAGTAGACACGTACAAGAACCAATTCTCCCTGAGGCTGATAT12050.10890135254576117No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA12000.10844947971362107No Hit
GGATAGCACCAGCTGGGAAAGGAGGATACCGAGGATACTACGACGGTTTG11920.10772648318219692No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC11910.10763610861576892No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC11780.10646123925220469No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG11580.10465374792364435No Hit
CCCCAGGAGTTCAGGTGCTGGGCACGGTGGGCATGTGTGAGTTTTGTCAC11560.1044729987907883No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG11470.10365962769293614No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA11460.10356925312650814No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA11410.10311738029436804No Hit
GTAGTAGTTACTACTGGGGCTGGATCCGACAGCCCCCAGGGAAGGGACTG11350.10257513289579993No Hit
TGCCCACCGTGCCCAGCACCTGAACTCCTGGGGGGACCGTCAGTCTTCCT11250.10167138723151975No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT11100.10031576873509948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA17400.073.389911
GGTATCA7200.071.1439741
ATCAACG22250.056.990863
TCAACGC22350.056.7358674
CAACGCA22800.055.2925765
TATCAAC23850.054.3759272
AACGCAG23400.053.2157826
TATTACG1101.05448635E-745.8032157
CGCAGAG28000.044.728248
CAGCGCT6900.040.6823589
GAATACG1807.8216544E-1139.9869359
GCAGAGT31600.039.4048429
ACGCAGA33250.037.4494177
GCAGCGC7500.036.4680828
GGCAGCG7650.035.753027
GTACACG15700.033.954375
TACACGG15950.033.394996
TATGTAC16450.033.2866632
CAGAGTA30800.032.716589
ACACGGG16650.032.4218377
Produced by FastQC (version 0.11.5)