FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1106506
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATATTACTGTGCGAGAGCCCTTAAGAGGATAGCACCAGCTGGGAAAGG52430.473833851782096No Hit
GTAATATACGGCCGTGTCCGCGGCGGTCACAGATATCAGCCTCAGGGAGA48850.44147975700086584No Hit
GTCTCTGGTGACTCCATCAGCAGTAGTAGTTACTACTGGGGCTGGATCCG43930.39701547031828116No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT33920.30655052932383553No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA29250.2643456068019514No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT28990.2619958680748229No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC28290.2556696484248617No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC28070.2536814079634453No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA27790.2511509201034608No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC25950.23452199988070557No Hit
GCCGTATATTACTGTGCGAGAGCCCTTAAGAGGATAGCACCAGCTGGGAA25180.22756315826574824No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG21340.19285932475738948No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT20820.18815984730313257No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG20410.18445449007958384No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT20010.18083950742246313No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19420.1755074080032101No Hit
ATATACGGCCGTGTCCGCGGCGGTCACAGATATCAGCCTCAGGGAGAATT18850.17035605771681311No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA18720.16918118835324888No Hit
CCTACAACCCGTCTCTCACGCCTCGACTCACCATATCAGTAGACACGTAC18360.16592770396184023No Hit
CACCTGCACTGTCTCTGGTGACTCCATCAGCAGTAGTAGTTACTACTGGG18320.16556620569612818No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT18290.16529508199684412No Hit
CTTTTATAGTGGGAGCACCTCCTACAACCCGTCTCTCACGCCTCGACTCA17140.1549020068576221No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT16610.15011215483693716No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT16540.14947953287194105No Hit
ATATTACTGTGCGAGAGCCCTTAAGAGGATAGCACCAGCTGGGAAAGGAG16310.14740091784409665No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC16080.14532230281625225No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG15630.14125544732699144No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG15300.13827308663486687No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT14530.13131424501990951No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC14520.1312238704534815No Hit
TCTGGGGCCAGGGGGCCACGGTCACCGTCTCCTCAGCCTCCACCAAGGGC14410.1302297502227733No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC14330.12950675369134917No Hit
CCATATCAGTAGACACGTACAAGAACCAATTCTCCCTGAGGCTGATATCT14250.12878375715992502No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA14230.12860300802706898No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC14020.12670514213208062No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA13740.12417465427209612No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC13740.12417465427209612No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC13630.12318053404138794No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC13310.12028854791569137No Hit
GTCATGGACCTCCTGTGCAAGAACATGAAGCACCTGTGGTTCTTCCTCCT13130.11866180571998707No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG13130.11866180571998707No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT13080.11820993288784697No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG12780.11549869589500646No Hit
GTCCCAACTACAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCGG12290.11107034214003358No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA12220.11043772017503746No Hit
AGTTACTACTGGGGCTGGATCCGACAGCCCCCAGGGAAGGGACTGGAGTG12140.10971472364361332No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT11980.10826873058076504No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG11880.10736498491648486No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG11860.10718423578362883No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC11600.10483449705650037No Hit
TAATTATGTATCCTGGTACCAGCAGCTCCCAGGATCAGCCCCCAAACTCC11430.10329812942722408No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCC11400.10302700572794002No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC11380.10284625659508398No Hit
GGATTGGGAATATCTTTTATAGTGGGAGCACCTCCTACAACCCGTCTCTC11270.1018521363643758No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC11090.10022539416867147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)