FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263040
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTACACTCTGGACGTCTGGGGCCAAGGGACCACGATCACCGTCTCCT45780.3624588294907525No Hit
GCCTACATGGACCTGAGCAGCCTGAGACCTGAGGACTCGGCCGTGTATTT44310.3508202432227008No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA43260.3425069673169496No Hit
CCCTATTACTCCTACACTCTGGACGTCTGGGGCCAAGGGACCACGATCAC40230.31851722827463896No Hit
GTGTAGGAGTAATAGGGTGGGTGAGGTTTACATGTCGCACAGAAATACAC40090.31740879148720547No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA38230.30268241702558907No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT35130.2781384595895617No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC34990.2770300228021282No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC33490.2651539143653408No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT31710.2510609323536864No Hit
CATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT31290.24773562199138588No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA30540.24179756777299213No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC30160.23878895363567265No Hit
GTAATAGGGTGGGTGAGGTTTACATGTCGCACAGAAATACACGGCCGAGT29780.2357803394983532No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG26670.21115720800608057No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG26120.20680263491259185No Hit
GGCCTAGAGTGGATGGGAGGGATCATCCCTGTCTTTGGTGCAGCAAAGTA24740.1958766151507474No Hit
ATGTAAACCTCACCCACCCTATTACTCCTACACTCTGGACGTCTGGGGCC23890.1891468203699012No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT22570.17869584494552823No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCTG22410.17742906004560424No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT21620.17117430960222954No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT21540.17054091715226755No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA21470.1699866987585508No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG21290.16856156574613632No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC21080.16689891056498607No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC20790.16460286293387383No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG20300.16072333417785659No Hit
CCCCTGGACAAGGCCTAGAGTGGATGGGAGGGATCATCCCTGTCTTTGGT20300.16072333417785659No Hit
CTATTACTCCTACACTCTGGACGTCTGGGGCCAAGGGACCACGATCACCG19930.15779389409678238No Hit
GAAATACACGGCCGAGTCCTCAGGTCTCAGGCTGCTCAGGTCCATGTAGG19410.15367684317202937No Hit
GTTACAGGTGTCCAGTCCCAGGTAAAGCTGGTGCAGTCTGGGGCTGAGGT19390.15351849505953888No Hit
CTGTTACAGGTGTCCAGTCCCAGGTAAAGCTGGTGCAGTCTGGGGCTGAG19210.1520933620471244No Hit
CCTTCAACACCTATGCTCTCGTCTGGGTGCGACAGGCCCCTGGACAAGGC19130.1514599695971624No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG18890.14955979224727642No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA18690.14797631112237142No Hit
GCCTGAGACCTGAGGACTCGGCCGTGTATTTCTGTGCGACATGTAAACCT18550.14686787433493792No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC18540.14678870027869267No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCTGTTACAGG17630.13958386116037497No Hit
ACATGGACCTGAGCAGCCTGAGACCTGAGGACTCGGCCGTGTATTTCTGT17620.13950468710412972No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT17520.1387129465416772No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG17500.13855459842918671No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT17080.13522928806688625No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC17020.13475424372941475No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG16990.134516721560679No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT16990.134516721560679No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT16770.13277489232328352No Hit
GTATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGA16700.13222067392956677No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA16690.13214149987332152No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT16490.13055801874841652No Hit
GGTTTACATGTCGCACAGAAATACACGGCCGAGTCCTCAGGTCTCAGGCT16200.12826197111730428No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC16170.12802444894856854No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG16030.12691601216113504No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC15800.1250950088674943No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15690.12422409424879656No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15420.12208639473017481No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT15410.12200722067392956No Hit
TCCCTGTCTTTGGTGCAGCAAAGTACGCACCGAGGTTCCAGGGCAGAGTC15230.12058208766151507No Hit
CTCTAGGCCTTGTCCAGGGGCCTGTCGCACCCAGACGAGAGCATAGGTGT15120.11971117304281732No Hit
GAGTAATAGGGTGGGTGAGGTTTACATGTCGCACAGAAATACACGGCCGA15040.11907778059285533No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC15030.11899860653661008No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC14850.1175734735241956No Hit
CCTATGCTCTCGTCTGGGTGCGACAGGCCCCTGGACAAGGCCTAGAGTGG14840.11749429946795034No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGCCG14560.11527742589308335No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG14500.11480238155561184No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA14450.1144065112743856No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT14260.11290220420572586No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA14190.11234798581200912No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA14170.11218963769951862No Hit
GTCCCAGGTAAAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGT13810.10933937167468964No Hit
GACCTGAGCAGCCTGAGACCTGAGGACTCGGCCGTGTATTTCTGTGCGAC13740.1087851532809729No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13560.10736002026855838No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG13260.10498479858120091No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG13240.1048264504687104No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA13200.10450975424372941No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG13040.10324296934380542No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC13030.10316379528756017No Hit
GTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCA12890.10205535850012669No Hit
TCTGGGGCCAAGGGACCACGATCACCGTCTCCTCTGCCTCCACCAAGGGC12830.10158031416265519No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC12750.10094692171269319No Hit
CACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA12750.10094692171269319No Hit
CCGCTGGTCAGGGCGCCTGAGTTCCACGACACCGTCACCGGTTCGGGGAA12710.1006302254877122No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)