FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1263040
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTACACTCTGGACGTCTGGGGCCAAGGGACCACGATCACCGTCTCCT43890.34749493286040034No Hit
GTGTAGGAGTAATAGGGTGGGTGAGGTTTACATGTCGCACAGAAATACAC41180.32603876361793765No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGTCCTCGGTGAAGGTCTCCTGCA38860.3076703825690398No Hit
GCCTACATGGACCTGAGCAGCCTGAGACCTGAGGACTCGGCCGTGTATTT38180.3022865467443628No Hit
CCCTATTACTCCTACACTCTGGACGTCTGGGGCCAAGGGACCACGATCAC37300.29531922979478087No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT36190.28653090955155813No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA33970.26895426906511277No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCC32880.26032429693438053No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA32050.25375285026602484No Hit
GTAATAGGGTGGGTGAGGTTTACATGTCGCACAGAAATACACGGCCGAGT31540.2497149733975171No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC31380.24844818849759312No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT29460.23324676969850522No Hit
CATGTAGGCTGTGCTCGTGGATTCGTCCGCGGTAATCGTGACTCTGCCCT28640.22675449708639472No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC27610.21859956929313404No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGG23450.1856631618951102No Hit
GTCCTGGTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT23170.18344628832024323No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG22480.177983278439321No Hit
GGCCTAGAGTGGATGGGAGGGATCATCCCTGTCTTTGGTGCAGCAAAGTA22250.17616227514568025No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCA21870.1731536610083608No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT21380.16927413225234356No Hit
GTGCAGGACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGTACTGCT20750.16428616670889284No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG19930.15779389409678238No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT19910.15763554598429186No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC19870.15731884975931087No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC19700.15597289080314164No Hit
CCTCTAAAGAAGCCCCTGGGAGCACAGCTCATCACCATGGACTGGACCTG19580.15502280212819863No Hit
GAAATACACGGCCGAGTCCTCAGGTCTCAGGCTGCTCAGGTCCATGTAGG19320.15296427666582213No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT19300.15280592855333164No Hit
CTATTACTCCTACACTCTGGACGTCTGGGGCCAAGGGACCACGATCACCG19050.1508265771472004No Hit
GCTCATCACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCTG19020.15058905497846467No Hit
ATGTAAACCTCACCCACCCTATTACTCCTACACTCTGGACGTCTGGGGCC18890.14955979224727642No Hit
CCCCTGGACAAGGCCTAGAGTGGATGGGAGGGATCATCCCTGTCTTTGGT17620.13950468710412972No Hit
CATAACAACCACATTCCTCCTCTAAAGAAGCCCCTGGGAGCACAGCTCAT17420.13792120597922472No Hit
GTATATGAGCAGCAGTAATAATCAGCCTCATCCTCAGCCTGGAGCCCAGA17280.13681276919179122No Hit
CTTGTACTCCTTGCCATTCAGCCAGTCCTGGTGCAGGACGGTGAGGACGC17140.13570433240435775No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCCGGGGAGCAGGG17060.13507093995439576No Hit
CCTTCAACACCTATGCTCTCGTCTGGGTGCGACAGGCCCCTGGACAAGGC17050.13499176589815048No Hit
CTGTTACAGGTGTCCAGTCCCAGGTAAAGCTGGTGCAGTCTGGGGCTGAG16830.13324993666075502No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC16700.13222067392956677No Hit
GTTACAGGTGTCCAGTCCCAGGTAAAGCTGGTGCAGTCTGGGGCTGAGGT16590.131349759310869No Hit
GGTTTACATGTCGCACAGAAATACACGGCCGAGTCCTCAGGTCTCAGGCT16490.13055801874841652No Hit
GAGTAATAGGGTGGGTGAGGTTTACATGTCGCACAGAAATACACGGCCGA16370.12960793007347352No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC15980.1265201418799088No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT15720.12446161641753231No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG15670.12406574613630607No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA15520.1228781352926273No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA15470.12248226501140105No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG15420.12208639473017481No Hit
ACCATGGACTGGACCTGGAGGTTCCTCTTTGTGGTGGCAGCTGTTACAGG15300.12113630605523182No Hit
GTCCTGCACCAGGACTGGCTGAATGGCAAGGAGTACAAGTGCAAGGTCTC15280.12097795794274131No Hit
CTCTAGGCCTTGTCCAGGGGCCTGTCGCACCCAGACGAGAGCATAGGTGT15160.12002786926779832No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT15070.11931530276159108No Hit
GCCTGAGACCTGAGGACTCGGCCGTGTATTTCTGTGCGACATGTAAACCT14690.11630668862427161No Hit
TCCCTGTCTTTGGTGCAGCAAAGTACGCACCGAGGTTCCAGGGCAGAGTC14610.11567329617430962No Hit
ACATGGACCTGAGCAGCCTGAGACCTGAGGACTCGGCCGTGTATTTCTGT14590.1155149480618191No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC14470.1145648593868761No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG14320.11337724854319736No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG14210.11250633392449962No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA14120.11179376741829238No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTCCTGCACCAGGACTGGCT13980.11068533063085888No Hit
GTCCAGGGGCCTGTCGCACCCAGACGAGAGCATAGGTGTTGAAGGTGTCT13620.1078350646060299No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG13450.10648910564986065No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA13450.10648910564986065No Hit
GTCCTTGACCAGGCAGCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGG13170.10427223207499367No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC12810.1014219660501647No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGTGTAGCCG12780.10118444388142894No Hit
GTCTTGGCATTATGCACCTCCACGCCGTCCACGTACCAGTTGAACTTGAC12720.10070939954395744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14400.067.4758451
TCAACGC15200.062.4320684
GGTATCA5650.061.146251
ATCAACG15650.060.1775253
CAACGCA15850.059.886015
AACGCAG16600.057.6272136
TATCAAC16800.056.060452
CGCAGAG20000.047.8324858
GCAGCGC8600.043.491688
CAGCGCT9400.039.790269
GGCAGCG10450.035.102497
ACGCAGA27600.034.6598437
GTATGTA19850.032.9957431
GTACACG19300.032.787335
ACACGGG19800.031.6037147
TATGTAC20550.031.1367152
ACGGGGT20150.029.6281389
GTACATG28600.028.689031
TATTGAG12550.028.0758575
ACATGGG28550.027.95083
Produced by FastQC (version 0.11.5)