FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1118368
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGCTTTGGAGATGGTTTT31790.28425348364760084No Hit
GTACGTGCTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCA31470.28139217144982687No Hit
GGTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCA31440.28112392343128556No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT30400.2718246587885204No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCC30320.27110933073907695No Hit
CTGTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCAC26340.23552176027926405No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGCGCCTGAGT25010.22362943145726627No Hit
GTACAACAGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGG23760.21245243068471203No Hit
CCTCCACGCCGTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGG21820.19510572548570776No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21690.1939433174053621No Hit
GTGGTGGACGTGAGCCACGAAGACCCTGAGGTCAAGTTCAACTGGTACGT20670.18482288477495779No Hit
GCCCAGGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGT20110.17981558842885348No Hit
GTGTATCTGCAAATGAACAGCCTGAAAACCGAGGACACAGCCGTTTATTT18960.16953274771810353No Hit
GTTGGAGACCTTGCACTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCA18220.16291596326075136No Hit
TCATGAGGGTGTCCTTGGGTTTTGGGGGGAAGAGGAAGACTGACGGTCCC17260.15433202666742968No Hit
GACCTACATCTGCAACGTGAATCACAAGCCCAGCAACACCAAGGTGGACA16940.1514707144696558No Hit
GGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCTGGGGAGCGGGG16740.1496823943460471No Hit
CCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGCCCTCCAGCAGC16170.14458568199376234No Hit
GATCAGGACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGA16130.1442280179690406No Hit
CCTTCCCGGCTGTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTG15950.14261852985779278No Hit
CCCTCATGATCTCCCGGACCCCTGAGGTCACATGCGTGGTGGTGGACGTG15430.1379688975364102No Hit
CCCGTAGACTCTCCTGTGCAGCCTCTGGATTCAGTTTCCCTAAGGCCTGG15250.13635940942516236No Hit
AGCACGTACCGTGTGGTCAGCGTCCTCACCGTTGTGCACCAGGACTGGCT15130.13528641735099717No Hit
GCGTGGAGGTGCATAATGCCAAGACAAAGCCGCGGGAGGAGCAGTACAAC15090.13492875332627544No Hit
GTTGTACTGCTCCTCCCGCGGCTTTGTCTTGGCATTATGCACCTCCACGC15090.13492875332627544No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14760.1319780251223211No Hit
GTCCTGGTGCACAACGGTGAGGACGCTGACCACACGGTACGTGCTGTTGT14750.13188860911614064No Hit
GCCCAGGGCCGCTGTGCCCCCAGAGGTGCTCTTGGAGGAGGGTGCCAGGG14280.12768605682566025No Hit
GTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAAAACCATCTCCAAAGC14100.12607656871441242No Hit
GCCCTGGGCTGCCTGGTCAAGGACTACTTCCCCGAACCGGTGACGGTGTC13880.12410941657844288No Hit
GTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT13790.12330467252281896No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTC13670.12223168044865375No Hit
CTCCTGTGCAGCCTCTGGATTCAGTTTCCCTAAGGCCTGGATGATGTGGG13580.12142693639302984No Hit
CTACAAGACCACGCCTCCCGTGCTGGACTCCGACGGCTCCTTCTTCCTCT13200.11802912815817333No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGGGAAAGAGCCACCCTCTCCT13190.1179397121519929No Hit
GTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTGT13080.11695613608400812No Hit
GTCAAGGACTACTTCCCCGAACCGGTGACGGTGTCGTGGAACTCAGGCGC12890.11525723196657987No Hit
GCTGAGGGAGTAGAGTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCA12890.11525723196657987No Hit
GGGCAGCGCTGGGTGCTTTATTTCCATGCTGGGCGCCCGGGAAGTATGTA12490.11168059171936251No Hit
ATGTAGGTCTGGGTGCCCAAGCTGCTGGAGGGCACGGTCACCACGCTGCT12460.1114123437008212No Hit
CTTGTACTCCTTGCCGTTCAGCCAGTCCTGGTGCACAACGGTGAGGACGC12430.1111440956822799No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT12410.11096526366991902No Hit
GACCAGCGGCGTGCACACCTTCCCGGCTGTCCTACAGTCCTCAGGACTCT12290.10989227159575381No Hit
CACCAGGACTGGCTGAACGGCAAGGAGTACAAGTGCAAGGTCTCCAACAA12170.1088192795215886No Hit
CCTCCAAGAGCACCTCTGGGGGCACAGCGGCCCTGGGCTGCCTGGTCAAG12040.10765687144124296No Hit
GAGTACAAGTGCAAGGTCTCCAACAAAGCCCTCCCAGCCCCCATCGAGAA12030.10756745543506253No Hit
GTCCACGTACCAGTTGAACTTGACCTCAGGGTCTTCGTGGCTCACGTCCA12000.10729920741652121No Hit
CGTCCACCACCACGCATGTGACCTCAGGGGTCCGGGAGATCATGAGGGTG11840.10586855131763427No Hit
GTCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGG11580.10354373515694297No Hit
GTGTACACCCTGCCCCCATCCCGGGATGAGCTGACCAAGAACCAGGTCAG11370.10166599902715384No Hit
GTACATGGGAGCTCTGGGAGAGGAGCCCCAGCCTTGGTGTTCCCAAGTGT11290.10095067097771038No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)