FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281488

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281488
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1017334
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT46750.45953443018713613No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33330.3276210172863583No Hit
ACATTACCCCGTGGACGTTCGGCCCAGGGACCAACGTGGAAGTCAAACGA31910.31366296614484523No Hit
GATCTATGGTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCATTG26220.2577324654439938No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT24590.24171019547169367No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTAGTAG22860.22470496415139965No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC22330.21949526900703212No Hit
GGGTAATGTAACTCTGCTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGC21610.21241794730147623No Hit
GAGTTACATTACCCCGTGGACGTTCGGCCCAGGGACCAACGTGGAAGTCA21080.2072082521571087No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT19350.19020302083681467No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG18860.18538651023164465No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG17810.17506541607770898No Hit
GTCCTGATCTATGGTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTT17480.17182164362932922No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG17460.17162505135973044No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG17430.17133016295533227No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15980.1570772234094211No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15690.15422663550023885No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA15150.14891864422107193No Hit
ATGTAACTCTGCTGACAGTAGTAAGTTGCAGAATCTTCAGGTTGCAGACT14870.1461663524466891No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT13700.13466570467516076No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG13070.12847304818279934No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG13000.12778497523920365No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA12500.12287016849923428No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG12450.12237868782523734No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12250.12041276512924957No Hit
CACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTACTACTGTCAGC12070.11864343470286061No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA11700.11500647771528329No Hit
GATTTCACTCTCACCATCAGCAGTCTGCAACCTGAAGATTCTGCAACTTA11670.11471158931088511No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC11650.11451499704128634No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA11390.11195929753650227No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC11270.11077974391890962No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACGTTGGTCCCTGGGCCGAACGTC11130.1094035980317182No Hit
ACTTACTACTGTCAGCAGAGTTACATTACCCCGTGGACGTTCGGCCCAGG11070.10881382122292188No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC11010.10822404441412554No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG10680.10498027196574576No Hit
TTACTACTGTCAGCAGAGTTACATTACCCCGTGGACGTTCGGCCCAGGGA10260.1008518343041715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14450.064.241151
TCAACGC15200.059.6070564
ATCAACG15400.058.8271453
CAACGCA15500.058.470635
AACGCAG16950.053.898386
TATCAAC18100.049.6545942
ACGCAGA19550.047.098277
CGCAGAG21050.044.0838288
GTACATG28950.036.2907261
TACATGG29150.034.7783132
CATTAGC10550.033.4106838
GCGTTAA900.00376352831.982241
ATTAGCA11100.031.7551969
ACATTAG11150.031.6127977
CTCCAAC11800.029.8567162
ACATGGG34150.028.6336143
CATGGGG18250.028.368764
GAATACG3301.8189894E-1228.3381259
GTCTTAG2853.5288394E-1027.774051
CAACATT13550.026.0109235
Produced by FastQC (version 0.11.5)