FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005281489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796275
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTACGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGAATAATCAAC33950.4263602398668802No Hit
GTAGTATACGGCCGTGTCCTCAGATTTCAGGCTGCTCAGCTCCATGTAGA32560.40890395905937016No Hit
GTCTACATGGAGCTGAGCAGCCTGAAATCTGAGGACACGGCCGTATACTA29270.36758657498979624No Hit
GTATACTACTGTGCGAGAGCCCTAACCCAATGTAGTAGTAGCAAATGTCC27620.34686509057800385No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC24380.3061756302784842No Hit
ATACCACACCACTCAAACTGTCATCCCATGCTGCACAGAAATAATCTGCC23710.2977614517597564No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG23250.29198455307525667No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAAA20940.2629744748987473No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC19580.24589494835326994No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT18770.23572258327839002No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAG18560.23308530344416187No Hit
GTATACGGCCGTGTCCTCAGATTTCAGGCTGCTCAGCTCCATGTAGACTG18350.23044802360993374No Hit
GCCGTATACTACTGTGCGAGAGCCCTAACCCAATGTAGTAGTAGCAAATG18300.22982009983987944No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCAC16790.21085680198423912No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT15280.1918935041285988No Hit
GTCTGGGGCTGAGGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA15230.1912655803585445No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14880.18687011396816428No Hit
CTATCTACACTGGGTACGACAGGCCCCTGGACAAGGGCTTGAGTGGATGG14680.18435841888794702No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC14630.1837304951178927No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC13220.166023044802361No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG13200.16577187529433926No Hit
ATCTACACTGGGTACGACAGGCCCCTGGACAAGGGCTTGAGTGGATGGGA13130.16489278201626323No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG12790.16062290037989388No Hit
GTTTAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGC12330.15484600169539417No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT12300.15446924743336157No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG12200.15321339989325294No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAT12030.15107845907506828No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG11960.15019936579699225No Hit
CCATGTAGACTGTGCTCGTGGATGTGTCCCTGGTCATGGTGACTCTGCCC11940.14994819628897052No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT11940.14994819628897052No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCA11680.14668299268468807No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG11460.1439201280964491No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG11380.14291545006436218No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC11150.14002700072211233No Hit
TATGTATACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT11150.14002700072211233No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA11140.1399014159681015No Hit
GTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCT11100.13939907695205803No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCA11010.13826881416596024No Hit
GAAATAATCTGCCTCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG10970.1377664751499168No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCACTCAAAC10940.13738972088788423No Hit
CATGTAGACTGTGCTCGTGGATGTGTCCCTGGTCATGGTGACTCTGCCCT10880.13663621236381904No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT10880.13663621236381904No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA10740.13487802580766695No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACA10590.13299425449750402No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTT10550.13249191548146055No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT10520.13211516121942796No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10450.13123606794135192No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA10350.1299802204012433No Hit
TCCAACAACCACATCCCTTCTCTACAGAAGCCTCTGAGAGGAAAGTTCTT10300.12935229663118897No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA10290.12922671187717813No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA10130.1272173558130043No Hit
CTACAGAAGCCTCTGAGAGGAAAGTTCTTCACCATGGACTGGACCTGGAG10070.12646384728893914No Hit
CTCTCAGGCCGGTCAGTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGC9860.12382656745471099No Hit
GGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCATCTATAGTAAT9840.12357539794668929No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCT9830.1234498131926784No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACAC9590.1204357790964177No Hit
CTCCCAGGTGCAGCTGGTGCAGTCTGGGGCTGAGGTGAAGAAGCCTGGGG9560.1200590248343851No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG9450.11867759254026561No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT9140.11478446516592884No Hit
CATCTATAGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTG9070.11390537188785282No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC8920.11202160057768988No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT8850.11114250729961384No Hit
GGGTTAGGGCTCTCGCACAGTAGTATACGGCCGTGTCCTCAGATTTCAGG8840.11101692254560296No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGA8810.11064016828357037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8520.10699821041725535No Hit
GTATCAACGCAGAGTACATGGGAGCTTCAGCTGTGGTAGAGAAGACAGGA8440.10599353238516843No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC8420.10574236287714672No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTG8370.10511443910709241No Hit
ACTATCTACACTGGGTACGACAGGCCCCTGGACAAGGGCTTGAGTGGATG8320.1044865153370381No Hit
ATGTAGTAGTAGCAAATGTCCTCTCAACTACTGGGGCCAGGGAACCCTGG8200.10297949828890773No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATC8040.10097014222473391No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG8040.10097014222473391No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)