FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences876550
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTTAGACCACCCCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAA26200.2988990930351948No Hit
CTTAAAAGGTGTCCAGTGTGAGGTGCAACTTTTGCAGTCTGGGGGAGGCT19780.2256574068792425No Hit
ATAATAGAGGGCCGTGTCCTCGGCTCGCAGGCTGTTCATTTCCAGATATA19430.22166448006388684No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT19270.21983914209115282No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC14800.16884376247789631No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13990.15960298899093034No Hit
CTATTATTGTGCGAAAGCCCAAGAAGCTTCAGGTCTCATAGAGTCATATT13010.14842279390793453No Hit
GGTGTACACCCTGCCCCCATCCCGGGAGGAGATGACCAAGAACCAGGTCA12820.1462552050653129No Hit
GTGTACACCTGTGGTTCTCGGGGCTGCCCTTTGGTTTTGGAGATGGTTTT12780.14579887057212937No Hit
CAATAATAGAGGGCCGTGTCCTCGGCTCGCAGGCTGTTCATTTCCAGATA11880.13553134447550053No Hit
GTATGTACACGGGGTACGTGCCAAGCATCCTCGCGCGACCCCGAGAGCCT11630.13267925389310364No Hit
GTATGCGTAGTGTGTGGTACCGCCATTGCCAGTAATTACTGAGATGCACT11240.1282299925845645No Hit
CTCTATTATTGTGCGAAAGCCCAAGAAGCTTCAGGTCTCATAGAGTCATA11210.12788774171467687No Hit
GTGTGTGGTACCGCCATTGCCAGTAATTACTGAGATGCACTCCAGCCCCT11050.12606240374194283No Hit
CTCCTAAGCTGCTCATTTACTGGGCATCTACCCGGGAATACGGGGTCCCT10660.1216131424334037No Hit
ACCACACACTACGCATACTCCGTGGAGGGCCGATTCACCGTCTCCAGAGA10230.11670754663168104No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10200.1163652957617934No Hit
CTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGAGCGCCTGAGT10120.11545262677542639No Hit
CTACGCATACTCCGTGGAGGGCCGATTCACCGTCTCCAGAGACAACTCCA9790.11168786720666248No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9590.10940619474074498No Hit
CCTTAGACCACCCCTCACTTTCGGCGGGGGGACCAAGGTGGAGATCAAAA9210.10507101705550168No Hit
GTCCTACAGTCCTCAGGACTCTACTCCCTCAGCAGCGTGGTGACCGTGAC9150.10438651531572643No Hit
TTATTACTGTCAGCAATACCTTAGACCACCCCTCACTTTCGGCGGGGGGA9130.10415834806913468No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9070.10347384632935941No Hit
GTCTAAGGTATTGCTGACAGTAATAAACTGCCACGTCTTCAGGCTGCAGG8990.10256117734299242No Hit
ATAGAGGGCCGTGTCCTCGGCTCGCAGGCTGTTCATTTCCAGATATAGCG8860.10107809024014602No Hit
GTGGTGGACGTGAGCCACGAAGACCCCGAGGTCCAGTTCAACTGGTACGT8830.1007358393702584No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC8770.10005133763048314No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19350.065.198121
GGTATCA8700.062.1469881
TCAACGC22100.056.7525524
ATCAACG22250.056.369953
CAACGCA22950.054.650615
AACGCAG23100.053.9806066
TATCAAC23800.053.304532
ACGCAGA25900.048.397
CGCAGAG26300.047.0847858
GCAGAGT31850.038.880069
GTACACG6350.036.324925
TACACGG6350.036.322856
ACACGGG6400.036.0143977
TATGTAC7700.030.8924032
GTACATG24500.029.1304471
GTATGTA9950.027.5320511
TACATGG25200.027.4599132
ACATGGG24150.027.1614343
ACATTAG5900.025.6373657
CATTAGC5950.025.4103248
Produced by FastQC (version 0.11.5)