FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281495

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281495
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences959585
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTATATGGAACTGAGCAGCCTGAGATCTGACGACACGGCTGTGTATTA27650.2881453961868933No Hit
GTAATACACAGCCGTGTCGTCAGATCTCAGGCTGCTCAGTTCCATATACA27290.2843937743920549No Hit
GTGTATTACTGTGCGAGAGGCATCTCTTCCGGCAAGTACGACTTTTTATC24170.25187971883678884No Hit
ATATGGAACTGAGCAGCCTGAGATCTGACGACACGGCTGTGTATTACTGT18410.19185377011937454No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT17350.1808073281679059No Hit
CATATACACTGTGCTCGCGGATGTGTCCCTGTAAACGGTGACTCTGCCCT15040.15673442165102622No Hit
GTCTGGGGCTGAAGTGAAGAAGCCTGGGGCCTCAGTGAAGGTTTCCTGCA14030.14620903828217405No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC13830.1441248039517083No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13070.13620471349593835No Hit
GTATATTGCCAACTGGTTAGCCTGGTATCAGCAGAAACCAGGGAAGGCCC12780.13318257371676298No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11030.11494552332518745No Hit
GTTCCATATACACTGTGCTCGCGGATGTGTCCCTGTAAACGGTGACTCTG10840.11296550071124498No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10560.11004757264859288No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT10480.10921387891640658No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC10370.10806755003465039No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG10370.10806755003465039No Hit
TCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAA10050.10473277510590516No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA9920.1033780227911024No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9770.10181484704325308No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG9650.1005643064449736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19500.066.211191
ATCAACG21600.058.6986273
CAACGCA21750.057.9595685
TCAACGC22000.057.303934
AACGCAG22950.055.1939476
TATCAAC24150.052.202332
ACGCAGA26500.047.528457
CGCAGAG27950.044.8052528
GAATACG3500.041.126669
CAGCCGT11000.037.9486929
GTGCTAG4450.037.2804221
ACAGCCG11650.037.0669448
GTAATAC11850.036.521271
AATACAC13500.032.017433
CGTACAC1153.2824615E-431.3214023
GGTATCA19600.030.912651
CCGATCG950.004899365430.3038549
CAGAGTA31500.029.9310679
GTACATG25250.029.4231281
CTAGAGA6400.029.2659324
Produced by FastQC (version 0.11.5)