FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281504

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281504
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55001271
Sequences flagged as poor quality0
Sequence length35-42
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG135150.012.7354835
TCTATAC91400.010.9670263
TACACTG149550.010.9159395
ATACTGT181050.010.4577036
TAGACTG116800.010.2698385
GTATAGA102600.010.09681
GTGTAGG125850.010.0356381
CCTACAC118500.09.9561153
TAGACAG150800.09.9547135
GTCCTAG116450.09.9317631
CTATACT119200.09.8535264
CTAGGAC104300.09.7296753
CTAGACT115850.09.5871214
CCTATAC70150.09.4839713
TAGGACC63500.09.33217054
CTAGTAC52500.09.2930873
CTACACT135400.09.2904654
CTTAGAC79700.09.1934463
TCTACAC108600.09.1483933
TAGGACT108050.09.1461934
Produced by FastQC (version 0.11.5)