FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281510

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281510
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60792856
Sequences flagged as poor quality0
Sequence length35-42
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG123400.012.6642775
TACACTG142850.011.0268745
TAGACCG27400.010.4230535
GTATAGG77050.010.1267191
TAGACTG115650.010.1078645
GTATAGA86850.010.0866261
ATACTGT156750.010.0379826
CTAGGAC113500.010.016363
CTACACT142050.09.9764874
TCTATAC83550.09.9132993
TAGACAG138000.09.8462155
GTCCTAG123450.09.7536561
CTAGACT108700.09.7086654
CCTACAC132500.09.6776553
CTATACT115750.09.6076824
TACACCG38250.09.5997195
CTAGTAC51200.09.4220783
GTCTAGA101400.09.3388211
TCTACAC111450.09.309443
CCTATAC70750.09.3002633
Produced by FastQC (version 0.11.5)