FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23329146
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG292690.12546108631666156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG32850.022.88937242-43
CTCGCTA51750.020.4095061
TAGCGCA120950.019.57980
TCGCTAT56900.018.9790332
TATCGCG8450.018.93283538-39
CCGATGT70300.017.43772332-33
CACCGAT71750.017.00194530-31
TATCTCG55300.016.4538938-39
CGCTATG67800.015.9866873
GTCACCG86150.014.16079328-29
TCTCGTA59650.014.0805740-41
GTATGCC62450.013.41717644-45
GACCGCG13200.013.3317845
ATGCCGT59750.013.2871646-47
CGTATCG11150.012.9135753
TATGCCG33700.012.58014144-45
AGCGCAG114550.012.257071580
CGCGTTC15450.012.1668988
GTCCGAT24050.011.9772291
GTCCGGT20450.011.9337091
Produced by FastQC (version 0.11.5)