FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39836930
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC427120.10721709730142356TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG75550.027.42274546-47
TCGTATG77200.025.69696842-43
CGTATGC95850.021.88614544-45
ATCGCGT11550.020.777240-41
CTCGCTA57400.019.7940391
TCGCTAT63500.017.6363472
GTATGCC127050.016.92075344-45
ATGCCGT123150.016.85576846-47
CGCTATG65800.016.59433
TCTCGTA120850.016.2665240-41
TAGCGCA151700.016.1374980
ATCTCGT128300.015.99221240-41
CGGAAGA375150.015.842734
CGTCTGA183350.015.619204516-17
TCGGAAG378400.015.442633
ATCGGAA376100.015.3457232
ACTTAGG194900.015.27824232-33
TGCCGTC140250.014.91471548-49
GATCGGA385750.014.9135451
GCCGTCT124700.014.89825548-49
Produced by FastQC (version 0.11.5)