FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281516

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281516
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38672184
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG48850.022.14703446-47
TCGTATG53950.020.57249542-43
CGCGTTC32850.017.8976258
CTCGCTA70350.017.743041
GACCGCG32750.017.3416295
TAGCGCA183400.016.68531880
TCGCTAT75900.016.283032
AGCGCAG201450.015.54772780
CCGCGTT39550.014.663397
GTCCGAT42250.014.3931031
CGCTATG86350.014.3587343
ACCGCGT40400.014.25589756
CGTATGC79350.013.93674544-45
CCCGTCT118450.013.5777731
CTCCGAT58950.013.3696471
CTCCGGT61600.012.8594391
TCCGATT58850.012.8449922
GTCCGTT35350.012.7887061
AGACCGC48050.012.5688764
CGTATCG23650.012.1763783
Produced by FastQC (version 0.11.5)