FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31987032
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG362670.11338032237564273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG80500.030.95344446-47
TCGTATG76250.029.08570142-43
GTCGTAT52900.026.6892842-43
CGTATGC103250.023.95887844-45
GTATGCC124350.020.71366144-45
ATGCCGT117650.020.44845646-47
TGTCGTA71500.019.63447240-41
CGGAAGA367200.019.5514344
TCGGAAG372750.019.0352043
CGTCTGA185350.019.02199416-17
AGAGCAC381050.019.0087688
ATCGGAA374550.018.9117182
GAGCACA387850.018.8404929
GATCGGA386100.018.2786981
TAGCGCA164700.017.89947380
ACGTCTG202200.017.56530814-15
TGCCGTC137750.017.36303748-49
GCCGTCT122200.017.16684248-49
ACACGTC208550.017.01121512-13
ATCGCGT8500.016.23373240-41
Produced by FastQC (version 0.11.5)