FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281518

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281518
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33934358
Sequences flagged as poor quality0
Sequence length86
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG375210.11056935274862131No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46400.023.40290546-47
ATCGCGT14650.022.7964640-41
TCGTATG46500.021.76134742-43
TAGCGCA179650.017.6793780
CTCGCTA62850.017.569211
TCGCTAT70750.016.1683522
CGCGTTC28950.015.3353018
AGCGCAG186400.015.04338880
GACCGCG29950.014.9567695
CGCTATG77700.014.4647473
CGTATGC75000.014.34527644-45
CGCGTAT17000.013.99865442-43
GTCCGTT32250.013.6461891
CGTCTGA158050.013.12150616-17
GTATGCC90700.013.11890344-45
CCGATTG42550.012.9719453
CGATTGG37500.012.9053774
GTCCGAT45850.012.8270411
TCGCGTA21250.012.42234340-41
TCTCGTA86700.012.40942640-41
Produced by FastQC (version 0.11.5)