FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281520

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281520
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27928162
Sequences flagged as poor quality0
Sequence length86
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGC659950.23630269689784816TruSeq Adapter, Index 11 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG90000.032.90797846-47
TCGTATG95050.031.18064542-43
CGGCTAC105250.028.90006832-33
ACGGCTA106650.028.42693932-33
CGTATGC115650.025.62661444-45
AGTCACG122800.024.99864828-29
TCTCGTA120550.024.20346340-41
GCCGTCT123400.023.91992648-49
ATGCCGT125400.023.71384846-47
GTATGCC128050.023.59790844-45
ATCTCGT127250.022.85051740-41
TGCCGTC133200.022.40026948-49
TCACGGC142750.021.39231530-31
CACGGCT145450.021.1189530-31
GTCACGG148000.020.9177928-29
CTCGTAT146150.020.88068642-43
CGGAAGA299100.020.512964
ATCGGAA297100.020.395742
TCGGAAG299100.020.2459143
CCGTCTT149900.019.82466350-51
Produced by FastQC (version 0.11.5)