FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19406763
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3038041.5654542697306089No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG471970.2431987240736644No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA402660.20748437026824104No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA339870.17512967000215338No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA339500.17493901481663893No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG218390.11253293503919228No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA216450.11153328352595433No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1157500.049.352421
AGCAGTG1213650.047.0047232
TCAACGC1223500.046.61528412
ATCAACG1226600.046.5583511
CAACGCA1226800.046.54623813
GTATCAA1231800.046.3724759
TATCAAC1233900.046.2769410
TGGTATC1233900.046.1739857
GTGGTAT1238900.046.0184676
GGTATCA1242650.045.887568
AGTGGTA1244350.045.845095
AACGCAG1246000.045.84477214
AGAGTAC1241300.045.8066819
ACGCAGA1251400.045.6453415
CAGTGGT1249800.045.5759664
GTACTTT1226800.045.45575722
CAGAGTA1258550.045.2746118
CGCAGAG1263450.045.1887816
GCAGAGT1262000.045.1199517
GAGTACT1253150.044.88883220