FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16742274
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3221341.9240755467268067No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA425670.2542486164065885No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG416910.24901635225895838No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA358040.21385386477368604No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA344280.20563514848699763No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG225490.1346830185672508No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA206070.12308363845914838No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG195980.11705697804252875No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT178490.10661036846010286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1187950.049.827011
AGCAGTG1241900.047.6118552
ATCAACG1271750.046.4655311
TCAACGC1271750.046.4017712
CAACGCA1274550.046.34371613
GTATCAA1280550.046.1616639
TGGTATC1277850.046.1327367
TATCAAC1282800.046.0989310
CAGTGGT1283000.045.9832954
AGTGGTA1284500.045.962655
GTGGTAT1283700.045.9384276
GGTATCA1287150.045.868068
AACGCAG1289850.045.81016514
AGAGTAC1287550.045.77126319
ACGCAGA1295000.045.63213315
GTACTTT1272800.045.37968422
CAGAGTA1302500.045.3191818
CGCAGAG1306800.045.20219816
GCAGAGT1305550.045.15450317
GAGTACT1295850.044.96473720