FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21608351
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT58500.017.54611
GGTATTG66950.017.438511
TTTATGC58300.016.9885271
GGGACCT57250.016.7234381
TGATTTC60700.016.7123851
CGACCTT66400.016.6638811
CTAATGG57050.016.6593131
GGGAATG37100.016.6110061
ATGAATG55300.016.5831310
CAATGAA62900.016.5828028
AGCTTGA59400.016.5728721
CTTTGCG57100.016.5571211
TACTTGT63450.016.5556721
GATGCTA54850.016.5432111
ACCTTTA86800.016.539461
GTACTTT72750.016.5157831
CCGGGAT49650.016.5026531
TCTATTG60550.016.4233361
CGGACTG53450.016.4150051
GTATATG43100.016.3582238
Produced by FastQC (version 0.11.5)