FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21137702
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3415411.6157905906706416No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG481850.22795760863692752No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA448120.21200033948818087No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA376530.17813194641498872No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA363910.17216157177350688No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG234530.11095340449023265No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAA234350.11086824859201819No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1254250.049.2881361
AGCAGTG1302050.047.424462
ATCAACG1334050.046.21221511
TCAACGC1334100.046.19899712
GTATCAA1335000.046.1893929
CAACGCA1338450.046.11356413
TGGTATC1337000.046.018287
TATCAAC1343500.045.903410
GGTATCA1348700.045.70628
GTGGTAT1350900.045.5955666
CAGTGGT1353350.045.5188674
AGAGTAC1352600.045.43047319
AACGCAG1359350.045.40351514
AGTGGTA1361200.045.292335
ACGCAGA1367550.045.13148515
GTACTTT1336250.045.0416922
GCAGAGT1366600.045.04142817
CAGAGTA1371100.044.92603318
CGCAGAG1382700.044.61694716
GAGTACT1365150.044.4873420