FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281609

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281609
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21395815
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATTG63300.017.5884111
ATGCGAT56900.017.5625821
TGATTTC63800.017.1683521
CTTTGCG57450.016.9243281
CCGGGAT47450.016.86511
GTACTTT70450.016.8540741
TTTATGC57850.016.8073041
ACCTTTA83700.016.7913671
ATGAATG52600.016.72898910
CGGACTG54050.016.6934471
GGGACCT58700.016.6832161
TCTATTG61800.016.607111
CTGATAG62500.016.5971661
CTCGAAA57650.016.5359361
TACTTGT63400.016.5193371
GATGCTA57400.016.5033951
TATTGGT38450.016.3366853
GTATATG45300.016.3122888
AGCTTGA56150.016.3007091
GGAACTT54800.016.3006941