FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18876298
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2395251.2689193612010152No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG384530.20371049450480175No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA288300.15273121880148322No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA257600.13646743657045465No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA227460.12050032267979663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT868250.048.1601451
AGCAGTG905100.046.139732
TCAACGC909900.045.83498812
CAACGCA911350.045.81936613
ATCAACG912300.045.79002811
GTATCAA919100.045.5015649
TGGTATC921200.045.2577367
TATCAAC925350.045.21767810
GTGGTAT926050.045.0766226
GGTATCA931450.044.8249748
AACGCAG932700.044.7810914
AGAGTAC931700.044.6967519
ACGCAGA935950.044.62813615
CAGTGGT940700.044.335754
AGTGGTA942900.044.2638055
GTACTTT924200.044.16943722
CAGAGTA948250.043.98169718
GCAGAGT947700.043.94025817
CGCAGAG950800.043.92564816
GAGTACT946950.043.50376520
Produced by FastQC (version 0.11.5)