FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19019791
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2388531.2558129581970694No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG430490.22633792348191417No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA293370.1542445971146581No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA255370.13426540806888992No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA239750.12605290983481365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT873450.048.0016371
ATCAACG911800.045.98149511
TCAACGC910750.045.9759912
AGCAGTG911550.045.9595152
CAACGCA912350.045.91390213
GTATCAA918350.045.6596769
TGGTATC921300.045.393397
TATCAAC925150.045.34922810
GTGGTAT927900.045.1199536
GGTATCA928950.045.08738
AACGCAG932050.044.98728614
ACGCAGA934300.044.85403415
AGAGTAC930550.044.82507719
CAGTGGT942600.044.4158334
AGTGGTA943600.044.396895
GTACTTT922500.044.34803422
CGCAGAG947050.044.23668316
CAGAGTA949150.044.06906518
GCAGAGT948650.044.0099917
GAGTACT941350.043.82537520
Produced by FastQC (version 0.11.5)