FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18996228
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510991.3218361034622241No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG453900.23894217315142777No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA294220.15488338000575694No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA251470.13237891227668988No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA224300.11807607278666059No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT888250.048.2020151
AGCAGTG928200.046.0251242
TCAACGC928350.045.9585512
CAACGCA930750.045.87065513
ATCAACG930900.045.84929711
GTATCAA937200.045.5359049
TGGTATC936950.045.4469387
TATCAAC942750.045.28449610
GTGGTAT944950.045.0859386
GGTATCA947500.045.001528
AACGCAG953100.044.830514
AGAGTAC947800.044.80023619
ACGCAGA958700.044.5905715
AGTGGTA960600.044.3957755
CAGTGGT961700.044.3233644
GTACTTT944800.044.0979622
CAGAGTA966750.044.0647518
GCAGAGT968000.043.971617
CGCAGAG972650.043.94116616
GAGTACT960150.043.80431720
Produced by FastQC (version 0.11.5)