FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26181039
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2729881.042693530993938No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG798270.30490386573275413No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA385510.14724778493321064No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA344570.13161051400595675No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325250.12423112772567964No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA263780.10075230398610231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1173400.048.361071
AGCAGTG1281600.044.273252
CAGTGGT1504450.037.6444474
TCAACGC1516600.037.3388712
CAACGCA1520800.037.28248613
GTATCAA1523800.037.164729
ATCAACG1528250.037.0830811
TGGTATC1525100.037.077997
AGTGGTA1531900.036.983125
TATCAAC1531950.036.97810410
GTGGTAT1532200.036.925486
GGTATCA1536050.036.8541568
AGAGTAC1540800.036.6750719
GCAGTGG1549550.036.6480943
AACGCAG1549150.036.60188314
ACGCAGA1553000.036.5159615
GTACTTT1530850.036.30695322
GCAGAGT1559300.036.2718417
CAGAGTA1564750.036.20353718
CGCAGAG1574450.035.99714316
Produced by FastQC (version 0.11.5)