FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25749977
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2673351.0381951020771785No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG768920.2986099754574538No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA382780.14865255996150986No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA351960.1366836172319688No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT319760.1241787516936423No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA261600.10159232375236685No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1138950.048.470341
AGCAGTG1246350.044.2276882
CAGTGGT1474300.037.3620074
TCAACGC1482900.037.147512
CAACGCA1488100.037.04237413
TGGTATC1489450.036.9261677
AGTGGTA1492550.036.9088485
GTATCAA1493000.036.9062359
ATCAACG1494100.036.88848511
TATCAAC1497350.036.83063510
GTGGTAT1499200.036.7196246
GGTATCA1504100.036.6283458
AGAGTAC1510800.036.3967219
AACGCAG1515400.036.3873514
GCAGTGG1520500.036.34163
ACGCAGA1518750.036.30692715
GCAGAGT1523400.036.1013717
GTACTTT1497950.036.0729422
CAGAGTA1532900.035.93115618
CGCAGAG1539100.035.82842616
Produced by FastQC (version 0.11.5)