FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27488986
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2491210.9062575098259353No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1129750.4109827841594448No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA367430.133664442915428No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA347150.1262869427049801No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT286890.10436543566939864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1100150.048.0999071
AGCAGTG1198500.044.126462
CAGTGGT1382800.038.1843384
TCAACGC1385100.038.15696712
CAACGCA1387900.038.0841513
ATCAACG1393150.037.96107511
GTATCAA1395200.037.9192289
TGGTATC1398500.037.7477537
TATCAAC1402750.037.73097610
AGTGGTA1402700.037.6507075
GTGGTAT1407350.037.541486
GGTATCA1413000.037.4213268
AGAGTAC1411400.037.3627319
AACGCAG1418100.037.2968614
ACGCAGA1422250.037.1805815
GTACTTT1400300.036.91916722
GCAGAGT1428650.036.9068317
CAGAGTA1433800.036.8278318
CGCAGAG1442150.036.65865716
GCAGTGG1453900.036.438283
Produced by FastQC (version 0.11.5)