FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28100596
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT69400.017.1062771
CGGACTG81800.017.0563141
GGCGACT54800.016.8455581
CAATGGA85900.016.8212328
GTACTTT95350.016.740811
GGTATTG85750.016.6321981
CTGCCTA104900.016.5420071
TACTTGT78600.016.5418871
CTTTGCG75300.016.4964661
ACTGAAC77100.016.461581
TGCGGGT61300.016.4298381
CTAATGG91350.016.3899461
GGGAATG58950.016.3722291
TGACTAG84650.016.3520951
CGATTGA82350.016.3351421
ATGCGAT58450.016.3069461
ACTGATG67300.016.2728391
GATGAAA79850.016.2328361
GAAATGA88550.016.2305221
TGACGGC66000.016.2296731
Produced by FastQC (version 0.11.5)