FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005281831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28076034
Sequences flagged as poor quality0
Sequence length58
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2491010.8872371361282723No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1144970.40781044787166165No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA368110.1311118229875345No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA353350.1258546702144612No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT283920.10112539399261306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1098100.048.2076641
AGCAGTG1197800.044.1926882
CAGTGGT1389950.038.07084
TCAACGC1389800.038.0347912
CAACGCA1393000.037.96441713
ATCAACG1398700.037.8260711
GTATCAA1399950.037.790779
TGGTATC1401750.037.679367
TATCAAC1405100.037.66403610
AGTGGTA1408950.037.5223165
GTGGTAT1412800.037.412636
GGTATCA1415900.037.338898
AGAGTAC1416400.037.2588519
AACGCAG1424500.037.1706414
ACGCAGA1430250.037.03019315
GTACTTT1407700.036.83220322
GCAGAGT1440800.036.71598417
CAGAGTA1442950.036.69050618
CGCAGAG1450000.036.52966316
GCAGTGG1456900.036.390143