FastQCFastQC Report
Wed 1 May 2024
EGAF00005281959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36922231
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length58
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8918292.4154255467390366Clontech SMART CDS Primer II A (100% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2429620.6580371592388337No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1847690.500427506669356Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT668940.18117540080392217Clontech SMART CDS Primer II A (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)