FastQCFastQC Report
Wed 8 May 2024
EGAF00005281973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005281973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4448128
Total Bases35.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGTACGC116170526.116716964979425No Hit
GTTCCTCA111753625.12373744640442ABI Solid3 Adapter A (100% over 8bp)
TAAGTATG107579024.18523028114299No Hit
CCCAGGAT101471222.812113320479988No Hit
GTTCATCA219380.4931962389571523No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)