FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282013

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282013
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740120
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA2200.0127.6413351
TTTGCGA1000.0115.188922
GGTATTG2900.0114.211351
GTCGTAA2500.0109.444271
TACACGA2250.0108.8042451
TACGGTA2450.0108.738931
CTCTACG2400.0108.00421
GTGCATA2750.0107.349641
ACGAGGA2750.0107.349641
TGACTAG2250.0105.604121
TCGAATG1500.0105.575588
CTTTGCG3700.0105.0851751
AGTTAGG2550.0104.4464348
GGATTAC2150.0103.817991
ACGCCGA2500.0103.6840441
CATCGAA2300.0103.308371
AACGTTG2650.0103.2493061
GTATTGC1750.0102.847252
AGTACGC1750.0102.833368
CCTTCGA1550.0102.197531
Produced by FastQC (version 0.11.5)